{
  "abstract": "The onset of mitosis in animal cells is marked by nuclear envelope breakdown (NEBD), which requires coordinated disassembly of nuclear pore complexes (NPCs) and the nuclear lamina. Polo-like kinase 1 (Plk1/PLK-1) initiates NEBD through phosphorylation of NPC components, but mechanisms that spatially regulate PLK-1 between NPCs and the nuclear interior are unclear. Here, we identify the Caenorhabditis elegans TMEM209 homolog NPP-28 as a transmembrane nucleoporin that concentrates PLK-1 at the nuclear envelope while limiting its nuclear accumulation to promote NEBD. We identify docking sites within NPP-28 that mediate PLK-1 nuclear envelope-association. NPP-28 is phosphorylated by PLK-1 in vitro, and mutational analysis suggests that NPP-28-mediated docking facilitates NEBD, whereas phosphorylation prevents premature NEBD. Simultaneous loss of npp-28 and the transmembrane nucleoporin npp-12/gp210 exacerbates mitotic NEBD defects in early embryos and causes severe defects in germline development. Together, our findings identify NPP-28 as a spatial regulator of PLK-1 during mitosis with functional relationships with other conserved transmembrane nucleoporins depending on context.",
  "abstract_shown": true,
  "affiliations": [
    "Yale University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "V. Puccini de Castro"
    },
    {
      "name": "S. R. Barger"
    },
    {
      "name": "R. Azamfirei"
    },
    {
      "name": "T. Y. Su"
    },
    {
      "name": "R. A. Green"
    },
    {
      "name": "R. J. Biggs"
    },
    {
      "name": "F. E. Q. Moore"
    },
    {
      "name": "S. Lee"
    },
    {
      "name": "K. Oegema"
    },
    {
      "name": "P. Lara-Gonzalez"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Bahmanyar"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9e14mdqa4ypme.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.05.752757",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.752757v1.full",
  "id": "9e14mdqa4ypme",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.752757v1",
  "language": null,
  "licence": "CC-BY-ND",
  "licence_label": "CC BY-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1312|Nuclear Structure and Function"
    ]
  ],
  "openalex": "W7220672408",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.752757v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9e14mdqa4ypme.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:16Z",
      "native_id": "10.64898/2026.10.05.752757",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.05.752757v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "TMEM209/NPP-28 partitions PLK-1 between the nuclear envelope and nuclear interior during mitosis",
  "title_text": "TMEM209/NPP-28 partitions PLK-1 between the nuclear envelope and nuclear interior during mitosis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:16Z",
  "url": "https://research.draconitic.com/paper/9e14mdqa4ypme",
  "venue": "bioRxiv",
  "versions": []
}