{
  "abstract": "Aeromonas hydrophila is one of the main pathogens of Tilapia and has a wide range phenotype, making it difficult to prevent by a unique vaccine. And turning the majority of the treatment using antibiotics. The aim of this work was to predict vaccine candidate with potential efficacy to protect Oreochromis niloticus against Aeromonas hydrophila, applying bioinformatics tools in a reverse vaccinology approach. It was obtained 35 A. hydrophila genomes in the São Francisco Valley in Petrolina/PE/Brazil, as well as 24 A. hydrophila and 02 O. niloticus complete genomes available in Genbank. After identification of core genes of Aeromonas hydrophila and a subtractive genomics analysis between the O. niloticus genome and A. hydrophila genome, 32 of the 178 resulting proteins, were considered likely vaccine targets as they showed a high adherence probability (> 0.51) in the Vaxign tool. After BLAST analysis against the database of essential genes (DEG), 18 proteins showed defined criteria e-value (≤ 10- 6) and a relevant similarity with the proteins of pathogenic species from the genus Aeromonas. Were selected 10 proteins, based on the criteria of portability adhesion, molecular mass between 19 and 80 kDa and presence of one or no transmembrane helix. The analyses identified nine proteins [secreted (n = 8) and PSE (n = 1)] with immunological and novel properties as candidates for vaccine targets. Finally, in vitro/in vivo tests will be necessary to experimentally validate the results of this search.",
  "abstract_shown": true,
  "affiliations": [
    "Center for Open Access Genomic Analysis - CALAnGO, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, CEP 54.300-300, Petrolina, Pernambuco, Brazil.",
    "Programa de Pós Graduação em Ciências Veterinárias no Semiárido. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco, Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil.",
    "Federal Institute (Instituto Federal - IF), Maria Luzia de Araújo Gomes Cabral, 791 - João de Deus, Petrolina, PE, CEP 56309-680, Brazil.",
    "Programa de Pós Graduação em Ciência Animal. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil.",
    "Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.",
    "Microbiology and Parasitology, Institute of Biological Sciences and Natural Sciences, Universidade Federal do Triângulo Mineiro, Uberaba, Brazil.",
    "Universidade Federal da Bahia, Salvador, BA, CEP 40170-110, Brazil.",
    "Dept. of Molecular Biology of Livestock, Georg August University Göttingen, Burckhardtweg 2, Göttingen, 37077, Germany.",
    "Center for Open Access Genomic Analysis - CALAnGO, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, CEP 54.300-300, Petrolina, Pernambuco, Brazil. gisele.veneroni@univasf.edu.br.",
    "Programa de Pós Graduação em Ciências Veterinárias no Semiárido. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco, Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil. gisele.veneroni@univasf.edu.br.",
    "Programa de Pós Graduação em Ciência Animal. Campus de ciências agrárias, Universidade Federal do Vale do São Francisco (Univasf), Rodovia BR-407, KM 12 Lote 543 S/n Projeto de Irrigação Nilo Coelho, Petrolina, Pernambuco, Brazil. gisele.veneroni@univasf.edu.br."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Germana Karla de Lima Carvalho",
      "orcid": "0009-0009-7337-6192"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Leticia Stheffany de Barros Cunha",
      "orcid": "0000-0003-2501-919X"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Maria Luiza Evangelista de Brito",
      "orcid": "0000-0002-9233-4758"
    },
    {
      "affs": [
        4
      ],
      "name": "Ligia Carolina da Silva Prado",
      "orcid": "0000-0002-4974-8269"
    },
    {
      "affs": [
        5
      ],
      "name": "Siomar de Castro Soares",
      "orcid": "0000-0001-7299-3724"
    },
    {
      "affs": [
        6
      ],
      "name": "Vasco Ariston de Carvalho Azevedo",
      "orcid": "0000-0002-4775-2280"
    },
    {
      "affs": [
        7
      ],
      "name": "Bertram Brenig",
      "orcid": "0000-0002-7635-9656"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Mateus Matiuzzi da Costa",
      "orcid": "0000-0002-9884-2112"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "João José de Simoni Gouveia"
    },
    {
      "affs": [
        8,
        9,
        10
      ],
      "name": "Gisele Veneroni Gouveia",
      "orcid": "0000-0002-0438-094X"
    },
    {
      "affs": [
        8,
        9,
        10
      ],
      "name": "Gisele Veneroni Gouveia",
      "orcid": "0000-0003-1074-5711"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9e28sb5y70y5p.bib",
  "cited_by": 0,
  "doi": "10.1007/s42770-026-02127-z",
  "fulltext_url": "https://europepmc.org/articles/PMC13691019",
  "id": "9e28sb5y70y5p",
  "issns": [
    "1517-8382",
    "1678-4405"
  ],
  "landing_url": "https://europepmc.org/article/MED/42911516",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Aquaculture disease management and microbiota"
    ]
  ],
  "openalex": "W7223188002",
  "pdf_url": "https://europepmc.org/articles/PMC13691019?pdf=render",
  "pmcid": "PMC13691019",
  "pmid": "42911516",
  "published": "2026-10-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9e28sb5y70y5p.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42911516",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42911516"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Aquaculture disease management and microbiota",
      "subfield": "immunology"
    }
  ],
  "title": "Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).",
  "title_text": "Reverse vaccinology for development of vaccine against Aeromonas hydrophila infection in nile tilapia (Oreochromis niloticus).",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/9e28sb5y70y5p",
  "venue": "Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]",
  "versions": []
}