{
  "abstract": "Schizophyllum commune, commonly known as the split gill mushroom, is an edible fungus recognized as a source of bioactive compounds with potential applications in the food and biotechnology industries. In this study, protein-enriched fractions were isolated from the fruiting bodies of S. commune using ion-exchange chromatography coupled with a bioassay-guided approach. Cytotoxic activity was evaluated in HeLa cells using MTT and colony formation assays. Among the fractions, the protein-enriched fraction F2 exhibited antiproliferative activity with an IC50 value of 0.37 μg/mL and significantly inhibited colony formation, while showing minimal effects on normal HaCaT cells. Flow cytometry and fluorescence staining further supported the involvement of apoptosis in F2-treated HeLa cells. Chemical and proteomic characterization of the protein-enriched fraction F2 by SDS-PAGE and LC–MS/MS identified 64 protein groups. Gene ontology analysis revealed proteins involved in diverse biological processes, while physicochemical profiling demonstrated a heterogeneous protein composition with isoelectric points ranging from 4 to 7. These findings provide insight into the chemical and proteomic composition of protein-enriched fraction F2. The results support further investigation of S. commune-derived protein-enriched fractions as a source of bioactive proteins, particularly in bioactivity-guided fractionation studies.",
  "abstract_shown": true,
  "affiliations": [
    "King Mongkut’s University of Technology North Bangkok , , ,",
    "Kasetsart University , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Saranya Sedtananun",
      "orcid": "0000-0001-5211-0568"
    },
    {
      "affs": [
        0
      ],
      "name": "Lueacha Tabtimmai",
      "orcid": "0000-0002-1311-9030"
    },
    {
      "affs": [
        0
      ],
      "name": "Chanapat Butsukon"
    },
    {
      "affs": [
        0
      ],
      "name": "Chanikan Sonklin",
      "orcid": "0000-0003-0264-5796"
    },
    {
      "affs": [
        1
      ],
      "name": "Kiattawee Choowongkomon",
      "orcid": "0000-0002-2421-7859"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9e454n937ey0k.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c03766",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670856/?tool=EBI",
  "id": "9e454n937ey0k",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13670856",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Fungal Biology and Applications"
    ]
  ],
  "openalex": "W7215066101",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670856/pdf/?tool=EBI",
  "pmcid": "PMC13670856",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/9e454n937ey0k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:18Z",
      "native_id": "PMC:PMC13670856",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13670856"
    },
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c03766",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/40/60808/5443172/Chemical-and-Proteomic-Characterization-of"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Fungal Biology and Applications",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells",
  "title_text": "Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:39Z",
  "url": "https://research.draconitic.com/paper/9e454n937ey0k",
  "venue": "ACS omega",
  "versions": []
}