{
  "abstract": "The molecular chaperone DNAJB6b inhibits the disease-related self-assembly of amyloid peptides in an efficient and ATP-independent manner. The molecular basis for this action is not yet fully understood, but the low-complexity region of DNAJB6b as a stand-alone peptide displays its antiamyloid formation properties and an ability to form self-assembled structures. Here, we studied the role of phenylalanine residues within the low-complexity region of the intact chaperone to gain insight into the role of these aromatic amino acid residues in chaperone-amyloid interactions. We designed, expressed, purified, and tested eight variants with various numbers of phenylalanine residues within the low-complexity region substituted with either alanine or serine. We show that substituting only 3 of these residues significantly affects the amyloid inhibition activity of the chaperone as well as its self-assembling ability, while substituting more residues affects these functions in a dose-dependent manner. Furthermore, we show that substituting all the phenylalanine residues within the low-complexity region reduces the amyloid inhibition activity of intact DNAJB6b to the equivalent of the isolated C-terminal domain, indicating that most of the chaperone activity stems from the aromatic residues within the low-complexity region.",
  "abstract_shown": true,
  "affiliations": [
    "Lund University , , Naturvetarvägen 14 , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Timas Merkelis",
      "orcid": "0000-0001-6974-9851"
    },
    {
      "affs": [
        0
      ],
      "name": "Erik Golovtchenko"
    },
    {
      "affs": [
        0
      ],
      "name": "Kalyani Sanagavarapu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lovisa Björn"
    },
    {
      "affs": [
        0
      ],
      "name": "Cecilia Emanuelsson"
    },
    {
      "affs": [
        0
      ],
      "name": "Ulf Olsson"
    },
    {
      "affs": [
        0
      ],
      "name": "Sara Linse",
      "orcid": "0000-0001-9629-7109"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9ec2nq9rn61fv.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c07904",
  "fulltext_url": "https://europepmc.org/articles/PMC13689251",
  "id": "9ec2nq9rn61fv",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/41/63724/5444895/The-Abundance-of-Phenylalanine-in-the-DNAJB6b",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Heat shock proteins research"
    ]
  ],
  "openalex": "W7220463937",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c07904",
  "pmcid": "PMC13689251",
  "pmid": "42923613",
  "published": "2026-10-05",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/9ec2nq9rn61fv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c07904",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/41/63724/5444895/The-Abundance-of-Phenylalanine-in-the-DNAJB6b"
    },
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42923613",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923613"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Heat shock proteins research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "The Abundance of Phenylalanine in the DNAJB6b Linker Governs Its Role in Amyloid Suppression",
  "title_text": "The Abundance of Phenylalanine in the DNAJB6b Linker Governs Its Role in Amyloid Suppression",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/9ec2nq9rn61fv",
  "venue": "ACS Omega",
  "versions": []
}