{
  "abstract": "We present a protocol for imaging and quantifying chromatin DNA replication-associated signals in synchronized mammalian cells. We describe steps for cell-cycle enrichment, nucleoside analog-based metabolic labeling, and fluorescence detection. We then detail the procedure for dual-color STORM imaging and SR-Tesseler-based cluster analysis. It can be adapted to distinct questions in DNA replication biology by modifying synchronization schemes, pulse durations, labeling strategies, fluorescence detection channels, or analysis parameters. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.",
  "abstract_shown": true,
  "affiliations": [
    "National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China. Electronic address: xiaozhiguang@stu.pku.edu.cn.",
    "National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China. Electronic address: guanxd@stu.pku.edu.cn.",
    "National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China.",
    "School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address: qian.su@uts.edu.au."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhiguang Xiao"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaodong Guan"
    },
    {
      "affs": [
        2
      ],
      "name": "Yujie Sun"
    },
    {
      "name": "Mengling Zhang"
    },
    {
      "affs": [
        3
      ],
      "name": "Qian Peter Su"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9ez7v099n5qfr.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104882",
  "fulltext_url": "https://europepmc.org/articles/PMC13676695",
  "id": "9ez7v099n5qfr",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42842375",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7220834395",
  "pdf_url": "https://europepmc.org/articles/PMC13676695?pdf=render",
  "pmcid": "PMC13676695",
  "pmid": "42842375",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9ez7v099n5qfr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42842375",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42842375"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Protocol for quantifying chromatin DNA replication by multiplex labeling and single-molecule localization microscopy.",
  "title_text": "Protocol for quantifying chromatin DNA replication by multiplex labeling and single-molecule localization microscopy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/9ez7v099n5qfr",
  "venue": "STAR protocols",
  "versions": []
}