{
  "abstract": "Microtubule minus ends are central to non-centrosomal microtubule organization, yet how their recognition, protection and molecular organization are integrated remains unclear. Here we show that Drosophila Patronin forms dynamic condensates at microtubule minus ends through a prion-like domain (PrLD). PrLD-mediated condensation cooperates with the CKK domain and a second CKK-independent recognition region to convert weak or broadly distributed microtubule-binding activities into persistent minus-end recognition and tracking. Replacing the PrLD with a heterologous phase-separating HNRNPA1 IDR restores minus-end targeting, indicating that condensation capacity rather than PrLD primary sequence is critical. Patronin condensates further form a protective cap that resists minus-end depolymerization and recruit the cytoskeletal regulators Sas-4, Katanin and Ena. Disrupting PrLD multivalency impairs Patronin localization at multiple developmental sites and causes early organismal lethality. Thus, Patronin condensation creates a physiologically essential microtubule minus-end compartment coupling end recognition, stabilization and cytoskeletal organization.",
  "abstract_shown": true,
  "affiliations": [
    "MOE Key Laboratory of Model Animals for Disease Study, Department of Laboratory Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing Univer"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "B. Zhang"
    },
    {
      "name": "J. Yang"
    },
    {
      "name": "S. Yu"
    },
    {
      "name": "K. Zhou"
    },
    {
      "name": "L. He"
    },
    {
      "name": "L. Huang"
    },
    {
      "name": "X. Liang"
    },
    {
      "name": "H. Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "J. Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9fnxf8k086rn0.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.03.756418",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.03.756418v1.full",
  "id": "9fnxf8k086rn0",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.03.756418v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W7220744527",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.03.756418v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-03",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9fnxf8k086rn0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:09Z",
      "native_id": "10.64898/2026.10.03.756418",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.03.756418v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Patronin condensation integrates recognition, protection and organization of microtubule minus ends",
  "title_text": "Patronin condensation integrates recognition, protection and organization of microtubule minus ends",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:09Z",
  "url": "https://research.draconitic.com/paper/9fnxf8k086rn0",
  "venue": "bioRxiv",
  "versions": []
}