{
  "abstract": "Intracellular lipopolysaccharide activates caspase-4/5 triggering pyroptotic cell death. We present a protocol for a high-throughput loss-of-function screening approach to identify interferon-gamma-stimulated genes regulating this pathway. We detail procedures for cell reverse-transfection with a custom-made small interfering RNA (siRNA) library in 384-well plates and priming with interferon-gamma. We then describe steps for transfection with lipopolysaccharide to induce pyroptosis and live-cell fluorescence microscopy using the IncuCyte S3 system to quantify cell death in real time without fixation. This approach enables reliable, high-throughput screening of cell death.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Intracellular Immunity, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw, Poland; Doctoral School of Molecular Biology and Biological Chemistry, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw, Poland. Electronic address: abajger@ibb.waw.pl.",
    "Laboratory of RNA Biology, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw, Poland; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.",
    "Laboratory of RNA Biology, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw, Poland.",
    "Laboratory of Intracellular Immunity, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw, Poland. Electronic address: mwandel@ibb.waw.pl."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Aleksandra Bajger"
    },
    {
      "affs": [
        1
      ],
      "name": "Lukasz S Borowski"
    },
    {
      "affs": [
        2
      ],
      "name": "Roman J Szczesny"
    },
    {
      "affs": [
        3
      ],
      "name": "Michal P Wandel"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9g543mwjvg8ay.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104884",
  "fulltext_url": "https://europepmc.org/articles/PMC13676704",
  "id": "9g543mwjvg8ay",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42848516",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Inflammasome and immune disorders"
    ]
  ],
  "openalex": "W7220931715",
  "pdf_url": "https://europepmc.org/articles/PMC13676704?pdf=render",
  "pmcid": "PMC13676704",
  "pmid": "42848516",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9g543mwjvg8ay.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42848516",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42848516"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Inflammasome and immune disorders",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for high-throughput identification of genes involved in pyroptotic cell death by live microscopy.",
  "title_text": "Protocol for high-throughput identification of genes involved in pyroptotic cell death by live microscopy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/9g543mwjvg8ay",
  "venue": "STAR protocols",
  "versions": []
}