{
  "abstract": "VP28, a major envelope protein of White Spot Syndrome Virus (WSSV), plays a critical role in viral entry and infection, making it an important target for developing novel control measures against this globally devastating shrimp pathogen. This study developed a non-GMO oral shrimp vaccine by externally immobilizing the WSSV VP28 protein on the surface of food-grade Pediococcus pentosaceus BT520 (Pp). VP28 was fused to the C-terminal domain of Lactococcus lactis N-acetylmuramidase AcmA (cA), a well-characterized cell wall binding module, and the resulting fusion protein was expressed, purified, and immobilized onto Pp cells. Surface display was confirmed by Western blot, flow cytometry and immunofluorescence microscopy. Its specific interaction with the shrimp Rab7 protein was also confirmed, and approximately 105 cA domains were estimated per bacterial cell. The VP28-displaying cells (Pp-VP28) remained stable in 25 ppt artificial seawater for up to 24 h. Oral administration of Pp-VP28 at 108 CFU g−1 feed to Penaeus vannamei (Pacific whiteleg shrimp) for 7 days yielded a relative percent survival (RPS) of 76.25 ± 3.75%, demonstrating marked protection against WSSV. These results highlight the potential of this non-GMO display platform for practical, scalable oral vaccination in sustainable shrimp aquaculture.",
  "abstract_shown": true,
  "affiliations": [
    "National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)",
    "Aquatic Animal Health Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)",
    "Department of Biology, Faculty of Science, Srinakharinwirot University",
    "Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Pinpunya Riangrungroj"
    },
    {
      "affs": [
        1
      ],
      "name": "Suparat Taengchaiyaphum"
    },
    {
      "affs": [
        0
      ],
      "name": "Somphop Boonpayung"
    },
    {
      "affs": [
        2
      ],
      "name": "Siwaporn Longyant"
    },
    {
      "affs": [
        3
      ],
      "name": "Dujduan Waraho-Zhmayev"
    },
    {
      "affs": [
        1
      ],
      "name": "Kallaya Sritunyalucksana"
    },
    {
      "affs": [
        0
      ],
      "name": "Ubolsree Leartsakulpanich"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9ge2ebmpzfpay.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-63552-6",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-63552-6",
  "id": "9ge2ebmpzfpay",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-63552-6",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2403|Aquaculture disease management and microbiota"
    ]
  ],
  "openalex": "W7170180443",
  "pdf_url": "https://www.nature.com/articles/s41598-026-63552-6_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/9ge2ebmpzfpay.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:53Z",
      "native_id": "71b216671af544cdba17a42b531ff1f2",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-63552-6"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Aquaculture disease management and microbiota",
      "subfield": "immunology"
    }
  ],
  "title": "Non-GMO surface display of VP28 on Pediococcus pentosaceus for oral protection against white spot syndrome virus in shrimp",
  "title_text": "Non-GMO surface display of VP28 on Pediococcus pentosaceus for oral protection against white spot syndrome virus in shrimp",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:53Z",
  "url": "https://research.draconitic.com/paper/9ge2ebmpzfpay",
  "venue": "Scientific Reports",
  "versions": []
}