{
  "abstract": "Pancreatic cancer is one of the major causes of cancer death. Pancreatic cancer cell lines are important for drug and cancer treatment development. In a collaborative effort, we established a novel Cancer Genome-in-a-Bottle cell line HG008-T from a pancreatic cancer patient explicitly consented to make public extensive genomic data, which was recently published. The HG008-T cell line is publicly available at ATCC as CRL-3734. Characterizing a cell line before it is available from a public repository is critical to ensure scientific reliability, and reproducibility. The cell line has known somatic variants in KRAS , TP53 , SMAD4 , and CDKN2A genes, and was derived from a patient who received platinum neoadjuvant therapy. To complement the extensive genomic characterization published recently, in this study we characterized HG008-T over 80 passages by comparing early-passage cells with late-passage cells. The cells were found to proliferate faster after extensive passaging and lost heterogeneity in morphology. Following extensive passaging, an increased proportion of cells exhibited whole-genome doubling (WGD), which may reflect the selective expansion of WGD clones due to enhanced proliferative capacity and/or de novo genome doubling events occurring in non-WGD cells during prolonged culture. The late-passage cells exhibited increased anchorage-independent growth. No significant surface marker expression alteration was observed. However, following approximately 80 passages, heterogeneous alterations in marker gene expression were observed. The late-passage cells were more sensitive to cisplatin but exhibited a modest shift in sensitivity to the CDK4/6 inhibitor palbociclib. In conclusion, extensive passaging altered certain characteristics of HG008-T cells, therefore, passage history should be indicated in future studies using HG008-T cell line as a cancer cell model.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "Zhiyong He",
      "orcid": "0009-0002-4650-2826"
    },
    {
      "name": "Jerilyn R. Izac"
    },
    {
      "name": "Alexander A. Gooden"
    },
    {
      "name": "Edward Kwee"
    },
    {
      "name": "Andrew S. Liss"
    },
    {
      "name": "Justin M. Zook"
    },
    {
      "name": "Hua-Jun He"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9gpmzftf91mmf.bib",
  "cited_by": 1,
  "doi": "10.1038/s41598-026-73171-w",
  "fulltext_url": null,
  "id": "9gpmzftf91mmf",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-73171-w",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Pancreatic and Hepatic Oncology Research"
    ]
  ],
  "openalex": "W7214924522",
  "pdf_url": "https://www.nature.com/articles/s41598-026-73171-w_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/9gpmzftf91mmf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.1038/s41598-026-73171-w",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-73171-w"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Pancreatic and Hepatic Oncology Research",
      "subfield": "oncology"
    }
  ],
  "title": "Effects of extensive passaging on the characteristics of pancreatic ductal adenocarcinoma cancer cell line HG008-T",
  "title_text": "Effects of extensive passaging on the characteristics of pancreatic ductal adenocarcinoma cancer cell line HG008-T",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:19Z",
  "url": "https://research.draconitic.com/paper/9gpmzftf91mmf",
  "venue": "Scientific Reports",
  "versions": []
}