{
  "abstract": "Tomato brown rugose fruit virus (ToBRFV), an emerging virus in the family Virgaviridae , threatens tomato production due to efficient seed and mechanical transmission. The use of plant growth-promoting rhizobacteria (PGPR) may offer an environmentally compatible approach to mitigating virus-associated symptoms and maintaining plant performance. This study evaluated two native bacterial isolates, Pseudomonas koreensis B1 and Acinetobacter calcoaceticus B2, applied individually and in combination (BMix), for their effects on ToBRFV symptom severity and growth, photosynthetic pigments, and antioxidant traits in the tomato cultivars Super Majar and Chocolate. Bacterial treatments were applied by seed priming and root drenching before ToBRFV inoculation, while the BMix treatment was also applied after viral inoculation to compare application timing. The experiment was conducted using a factorial randomized complete block design. Five independent biological replicates were used for disease-severity assessment and three for growth, pigment, and antioxidant measurements. Bacterial treatments were associated with reduced symptom severity and improvements in several growth, pigment, and antioxidant traits compared with the ToBRFV-inoculated control. The BMix + V treatment exhibited the lowest disease severity, with values of 15% and 20% in Super Majar and Chocolate, respectively, whereas V + BMix generally showed weaker responses. Treatment effects varied between cultivars, with Super Majar generally showing more favorable responses than Chocolate. Principal component analysis (PCA) further revealed separation among treatments based on their measured growth, pigment, and antioxidant traits. Overall, these findings indicate that the tested native PGPR isolates, particularly when applied in combination before viral inoculation, may help reduce ToBRFV symptom severity and maintain physiological performance in tomato plants under greenhouse conditions.",
  "abstract_shown": true,
  "affiliations": [
    "Plant Protection Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran",
    "Plant Protection Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran; Corresponding author.",
    "Qazvin Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Qazvin, Iran",
    "Department of Plant Production and Genetics, Faculty of Agriculture, Malayer University, Malayer, Iran"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sara Almasi"
    },
    {
      "affs": [
        1
      ],
      "name": "Samira Pakbaz"
    },
    {
      "affs": [
        2
      ],
      "name": "Ehsan Hasanvand"
    },
    {
      "affs": [
        3
      ],
      "name": "Reza Almasi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9hs2j6wtx5nkn.bib",
  "cited_by": 0,
  "doi": "10.1016/j.biocontrol.2026.106176",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S1049964426002203",
  "id": "9hs2j6wtx5nkn",
  "issns": [
    "1049-9644",
    "1090-2112"
  ],
  "landing_url": "https://doi.org/10.1016/j.biocontrol.2026.106176",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "S"
    ],
    [
      "openalex",
      "1110|Plant-Microbe Interactions and Immunity"
    ]
  ],
  "openalex": "W7223790886",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-31",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/9hs2j6wtx5nkn.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T14:22:57Z",
      "native_id": "1051d20f3d91400ea84183fdf81b7b6a",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.biocontrol.2026.106176"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant-Microbe Interactions and Immunity",
      "subfield": "plant-biology"
    }
  ],
  "title": "Priming tomato seeds with native isolates of",
  "title_text": "Priming tomato seeds with native isolates of",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T14:20:22Z",
  "url": "https://research.draconitic.com/paper/9hs2j6wtx5nkn",
  "venue": "Biological Control",
  "versions": []
}