{
  "abstract": "Newcastle disease (ND), a major cause of economic loss in the poultry industry worldwide, is caused by outbreaks of virulent avian paramyxovirus type 1(APMV-1) virus. We previously demonstrated that chicken miRNA-27b-5p (gga-miR-27b-5p) can regulate the APMV-1 phosphoprotein (P) and alter viral replication. In this study, the LaSota vaccine strain of APMV-1 was used as a backbone to express the chicken pre-miRNA-27b and expected to increase intra-cellular levels of both gga-miR-27b-5p and gga-miR-27b-3p. This novel recombinant virus, rLS-miR-27b, was rescued, propagated in embryonating chicken eggs (ECEs) and did not exhibit toxicity in ECEs. Expression of both gga-miR-27b-5p and gga-miR-27b-3p was increased significantly relative to controls. An inverse correlation was observed between increased expression of gga-miR-27b-5p and reduced APMV-1 P transcripts. Additionally, increased expression of interferons (alpha, beta and lambda) and interferon stimulated gene MX1 was observed. Suppression of P did not alter the P:V ratio in rLS-27b virus infected cells. These data support the observation that miR-27b-5p expression alters viral transcriptional kinetics in cell culture without affecting overall progeny virus yield in ECEs and could be utilized to improve current APMV-1 vaccines.",
  "abstract_shown": true,
  "affiliations": [
    "Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratories, US National Poultry Research Center, 934 College Station Road, Athens, GA, 30605, United States. Electronic address: Abhijeet.Bakre@usda.gov.",
    "Core Sequencing Lab, US National Poultry Research Center, 950 College Station Road, Athens, GA, 30605, United States.",
    "Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratories, US National Poultry Research Center, 934 College Station Road, Athens, GA, 30605, United States."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Abhijeet Bakre"
    },
    {
      "affs": [
        1
      ],
      "name": "Timothy R Satterlee"
    },
    {
      "affs": [
        2
      ],
      "name": "Megan C Mears"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9jhn26v96xh6z.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107893",
  "fulltext_url": "https://europepmc.org/articles/PMC13681985",
  "id": "9jhn26v96xh6z",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://europepmc.org/article/MED/42863174",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Virology and Viral Diseases"
    ]
  ],
  "openalex": "W7220413975",
  "pdf_url": "https://europepmc.org/articles/PMC13681985?pdf=render",
  "pmcid": "PMC13681985",
  "pmid": "42863174",
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9jhn26v96xh6z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42863174",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42863174"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Virology and Viral Diseases",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.",
  "title_text": "A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/9jhn26v96xh6z",
  "venue": "Poultry science",
  "versions": []
}