{
  "abstract": "Introduction\n\nJanus kinase 3 (JAK3) is a non-receptor tyrosine kinase that phosphorylates multiple proteins, including β-catenin, a critical component of adherens junctions (AJs). We previously demonstrated that JAK3-mediated phosphorylation of β-catenin regulates its basolateral localization and preserves epithelial barrier integrity. However, the structural basis underlying JAK3-β-catenin interactions and their role in epithelial-mesenchymal transition (EMT) remains unclear.Methodology\n\nHere, we employed homology modeling, molecular docking, and structural simulations to investigate phosphorylation-dependent interactions between JAK3 and β-catenin.Results\n\nOur analysis revealed that Src-mediated phosphorylation at Y654 induces conformational remodeling of the β-catenin N-terminal domain (NTD), creating a favorable interface for JAK3 binding and shifting its interaction from the armadillo repeat domain to the NTD. Subsequent JAK3-mediated phosphorylation at Y30, Y64, and Y86 restores β-catenin conformation, stabilizes the E-cadherin/β-catenin complex, and limits nuclear translocation. Phosphomimetic analyses identified distinct structural contributions of these residues.Conclusions\n\nCollectively, these findings reveal a sequential Src-JAK3 phosphorylation mechanism that maintains AJ integrity and suppresses EMT, providing potential therapeutic insights into epithelial cancer progression.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Kingsville, TX 78363, USA."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jugal Kishore Das",
      "orcid": "0000-0002-4553-2196"
    },
    {
      "affs": [
        0
      ],
      "name": "Jayshree Mishra",
      "orcid": "0009-0005-7336-4977"
    },
    {
      "affs": [
        0
      ],
      "name": "Narendra Kumar",
      "orcid": "0000-0002-1193-0954"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9jnk2mgb2nej9.bib",
  "cited_by": 0,
  "doi": "10.3390/biology15191738",
  "fulltext_url": "https://europepmc.org/articles/PMC13663477",
  "id": "9jnk2mgb2nej9",
  "issns": [
    "2079-7737"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883412",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Wnt/β-catenin signaling in development and cancer"
    ]
  ],
  "openalex": "W7215090205",
  "pdf_url": "https://europepmc.org/articles/PMC13663477?pdf=render",
  "pmcid": "PMC13663477",
  "pmid": "42883412",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9jnk2mgb2nej9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42883412",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883412"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Wnt/β-catenin signaling in development and cancer",
      "subfield": "molecular-biology"
    }
  ],
  "title": "JAK3-Mediated Structural Remodeling of β-Catenin Suppresses Epithelial-Mesenchymal Transition: Insights from In Silico Modeling.",
  "title_text": "JAK3-Mediated Structural Remodeling of β-Catenin Suppresses Epithelial-Mesenchymal Transition: Insights from In Silico Modeling.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/9jnk2mgb2nej9",
  "venue": "Biology",
  "versions": []
}