{
  "abstract": "Introduction: Mammary epithelial cells are active participants in local innate immunity, mounting NF-κB-dependent cytokine responses to inflammatory stimuli. Excessive or persistent activation of this pathway, however, can sustain inflammation. Resolvin D1 (RvD1) is a lipid mediator with pro-resolving activity, but the specific mechanism by which RvD1 regulates inflammation in bovine mammary epithelial cells remains to be elucidated.\n\nMethods: Using an LPS-induced inflammatory model in MAC-T bovine mammary epithelial cells, with selected responses confirmed at predefined time points in primary bovine mammary epithelial cells, this study investigated how RvD1 pretreatment attenuates subsequent LPS-induced inflammatory activation.\n\nResults: The results showed that RvD1 significantly inhibited LPS-induced mammary epithelial inflammation, and that its early anti-inflammatory effect involved FPR2/ARRB2-associated suppression of NF-κB signaling. Further investigation showed that ARRB2 contributed to the RvD1-mediated reduction in p65 and HDAC1 enrichment at the MEG3 promoter-proximal region and the restoration of H3K27ac at this region. Meanwhile, RvD1 increased CREB phosphorylation in an FPR2-sensitive manner and increased CREB enrichment at this region; CREB1 knockdown attenuated the RvD1-associated increases in MEG3 and DLK1–DIO3 locus-associated miRNAs. Among these miRNAs, bta-miR-380-5p and bta-miR-654 targeted TAB2 and IL-33, respectively, and contributed to the later suppression of IL-6 and IL-8 secretion by RvD1.\n\nDiscussion: These findings identify receptor-proximal, chromatin, and microRNA-associated mechanisms underlying the anti-inflammatory response to RvD1 in bovine mammary epithelial cells.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Northwest A&F University",
    "Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Naihan Yuan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yanfei Feng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhuo Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pengfei Lin"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Aihua Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yaping Jin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9p5ads3n697h1.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1978720",
  "fulltext_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978720/full",
  "id": "9p5ads3n697h1",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978720/full",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC581-607"
    ],
    [
      "openalex",
      "1102|Milk Quality and Mastitis in Dairy Cows"
    ]
  ],
  "openalex": "W7223265047",
  "pdf_url": "https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1978720/pdf",
  "pmcid": "PMC13739536",
  "pmid": "42996483",
  "published": "2026-10-23",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/9p5ads3n697h1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:17Z",
      "native_id": "10.3389/fimmu.2026.1978720",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978720/full"
    },
    {
      "first_seen": "2026-10-30T13:43:13Z",
      "native_id": "ef4e0f4b769e48b1b9d65a7af8fbdf4a",
      "source": "doaj",
      "url": "https://doi.org/10.3389/fimmu.2026.1978720"
    },
    {
      "first_seen": "2026-11-09T15:20:41Z",
      "native_id": "MED:42996483",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42996483"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Milk Quality and Mastitis in Dairy Cows",
      "subfield": "plant-biology"
    }
  ],
  "title": "Resolvin D1 engages FPR2–ARRB2 signaling and chromatin–microRNA regulation to restrain inflammatory activation in bovine mammary epithelial cells",
  "title_text": "Resolvin D1 engages FPR2–ARRB2 signaling and chromatin–microRNA regulation to restrain inflammatory activation in bovine mammary epithelial cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/9p5ads3n697h1",
  "venue": "Frontiers in Immunology",
  "versions": []
}