{
  "abstract": "Background\n\nTesticular torsion is a urological emergency that may result in irreversible ischemia-reperfusion injury and longterm impairment of spermatogenic function despite timely surgical detorsion. Mitochondrial dysfunction plays a central role in oxidative stress-induced germ cell damage following torsion. This study aimed to investigate the protective effects of isolated intratesticular mitochondrial transplantation in a rat model of testicular torsion-detorsion injury.Methods\n\nThirty-six adult male Sprague-Dawley rats were randomly allocated into four groups: sham (n=4), torsion (n=10), torsion+vehicle (n=10), and torsion+mitochondria (n=10). Testicular torsion was induced by a 720° rotation for 2 h, followed by surgical detorsion. Isolated mitochondria were administered through intratesticular injection before torsion induction. Histopathological evaluation was performed using hematoxylin-eosin staining and quantified by the Johnsen scoring system. Apoptotic activity was assessed by Caspase-3 immunohistochemistry. Functional outcomes were evaluated through total sperm count, motile sperm count, and detailed sperm motility subtype analysis.Results\n\nThe torsion and vehicle groups demonstrated marked histological damage, significantly reduced Johnsen scores, and increased apoptotic activity compared with the sham group. Mitochondrial transplantation significantly preserved seminiferous tubular architecture and resulted in higher Johnsen scores compared with untreated torsion (9.7±0.4 vs. 6.4±1.0, p<0.001). Caspase-3 expression was significantly reduced in the mitochondria-treated group, approaching levels observed in sham animals. Functionally, total and motile sperm counts were significantly higher in mitochondria-treated rats than in torsion and vehicle groups. Detailed motility analysis revealed restoration of progressive sperm motility patterns, whereas progressive motility was completely absent in untreated ischemic groups.Conclusion\n\nIntratesticular mitochondrial transplantation provides significant structural and functional protection against ischemia-reperfusion injury in experimental testicular torsion. By preserving spermatogenic architecture, reducing apoptosis, and restoring progressive sperm motility, mitochondrial therapy represents a promising experimental strategy for fertility preservation in ischemic testicular disorders.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Urology, Gülhane Training and Research Hospital, Ankara-Türkiye.",
    "Department of Urology, Etlik City Hospital, Ankara-Türkiye.",
    "Department of Pharmacology, Ankara University Faculty of Pharmacy, Ankara-Türkiye.",
    "Department of Mitochondria and Cellular Research, Gülhane Health Sciences Institute, University of Health Sciences, Ankara-Türkiye.",
    "Department of Pathology, Gülhane Training and Research Hospital, University of Health Sciences, Ankara-Türkiye.",
    "Department of Urology, Bilkent City Hospital, Ankara-Türkiye.",
    "Institute of Transplantation and Gene Sciences, Başkent University, Ankara-Türkiye."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Cihat Ozcan"
    },
    {
      "affs": [
        1
      ],
      "name": "Adem Sancı"
    },
    {
      "affs": [
        2
      ],
      "name": "Umur Deniz Mendeş"
    },
    {
      "affs": [
        2
      ],
      "name": "Merve Sözer"
    },
    {
      "affs": [
        3
      ],
      "name": "Öner Ülger"
    },
    {
      "affs": [
        4
      ],
      "name": "Gülçin Şimşek"
    },
    {
      "affs": [
        4
      ],
      "name": "Muhammed Mustafa Ayturk"
    },
    {
      "affs": [
        0
      ],
      "name": "Alper Murat Albayrak"
    },
    {
      "affs": [
        2
      ],
      "name": "Serap Gür"
    },
    {
      "affs": [
        5
      ],
      "name": "Cevdet Serkan Gökkaya"
    },
    {
      "affs": [
        6
      ],
      "name": "Gökhan Burçin Kubat"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9pqys8nfeqxv0.bib",
  "cited_by": 0,
  "doi": "10.14744/tjtes.2026.02503",
  "fulltext_url": "https://europepmc.org/articles/PMC13678157",
  "id": "9pqys8nfeqxv0",
  "issns": [
    "1306-696X",
    "1307-7945"
  ],
  "landing_url": "https://europepmc.org/article/MED/42841884",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7220746010",
  "pdf_url": "https://europepmc.org/articles/PMC13678157?pdf=render",
  "pmcid": "PMC13678157",
  "pmid": "42841884",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9pqys8nfeqxv0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42841884",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42841884"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Mitochondrial transplantation attenuates apoptosis and improves spermatogenic function following testicular torsion-detorsion injury in rats.",
  "title_text": "Mitochondrial transplantation attenuates apoptosis and improves spermatogenic function following testicular torsion-detorsion injury in rats.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/9pqys8nfeqxv0",
  "venue": "Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES",
  "versions": []
}