{
  "abstract": "Background: Bulk transcriptomic profiles combine signals from malignant, stromal, and immune cells. This study examined immune-associated gene expression in prostate cancer using bulk and single-cell transcriptomic data, and separately assessed the effects of FLT3 knockdown in prostate cancer cell lines.\n\nMethods: We screened 502 sample records from 497 patients and identified genes shared by ImmuneScore-associated differential expression and a weighted gene co-expression network module. Candidate genes were compared with four functional gene sets. We analyzed associations between FLT3 expression and immune-related signatures, examined single-cell expression patterns, and assessed FLT3 knockdown using two independent siRNAs in DU145 and 22Rv1 cells.\n\nResults: The screening identified 64 shared genes, and comparison with the functional gene sets prioritized FLT3 and AIM2. FLT3 expression was associated with several immune-cell signatures; associations were stronger for total T-cell and B-cell signatures than for the CD8-positive T-cell signature. Single-cell analyses suggested FLT3 expression in cells annotated as monocytes. In DU145 and 22Rv1 cells, both FLT3-targeting siRNAs reduced growth and colony formation, reduced Transwell migration and invasion readouts, and altered epithelial–mesenchymal transition markers.\n\nConclusion: The tissue analyses support an association between FLT3 expression and immune-related transcriptional signals. The cell-line findings describe a separate in vitro phenotype and do not establish how FLT3 relates to lymphocyte infiltration. These findings do not establish a causal immune-regulatory mechanism or prognostic utility.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Urology, The Affiliated Hospital of Xuzhou Medical University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yongqiang Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Tianmou Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Xingyue Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zijin Wan"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijun Mao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9s7et9bcds55k.bib",
  "cited_by": 0,
  "doi": "10.3389/fonc.2026.1970520",
  "fulltext_url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1970520/full",
  "id": "9s7et9bcds55k",
  "issns": [
    "2234-943X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1970520/full",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC254-282"
    ],
    [
      "openalex",
      "2740|Prostate Cancer Treatment and Research"
    ]
  ],
  "openalex": "W7223361988",
  "pdf_url": "https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2026.1970520/pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/9s7et9bcds55k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:13Z",
      "native_id": "10.3389/fonc.2026.1970520",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fonc.2026.1970520/full"
    },
    {
      "first_seen": "2026-10-30T13:43:11Z",
      "native_id": "29ec89e617ce42a2b9731119f4675d65",
      "source": "doaj",
      "url": "https://doi.org/10.3389/fonc.2026.1970520"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:RC254-282",
      "subfield": "oncology"
    }
  ],
  "title": "FLT3 expression and immune-associated transcriptional signatures in prostate cancer",
  "title_text": "FLT3 expression and immune-associated transcriptional signatures in prostate cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:43:11Z",
  "url": "https://research.draconitic.com/paper/9s7et9bcds55k",
  "venue": "Frontiers in Oncology",
  "versions": []
}