{
  "abstract": "Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been established as a powerful tool for the spatial characterization of molecules. Recent advances have been driven by improvements in the spatial resolution, acquisition time, and sensitivity. Although MALDI-MSI excels in the label-free, multiplexed detection of biomolecules, variations in sample preparation remain a significant source of experimental variability. Here, we present a workflow demonstrating the integration of a robust, completely automated tissue section processing system into a MALDI workflow. Importantly, PFA fixation and agarose embedding are demonstrated to preserve lipid compositional distributions. Combined, it enables robust and stable lipid imaging across a completely sectioned mouse brain. The integration of the Marinus MP system advances mass-spectrometry imaging toward a fully automated workflow, significantly reduces sample-preparation variability, and enables more consistent, reliable data acquisition and improved biological interpretation. Parallel with sectioning, serial two-photon (STP) tomography is used to acquire high-resolution digital images of every single section, providing a structural reference for downstream analyses. We demonstrate how the Allen Mouse Brain Atlas can be seamlessly integrated with these multimodal imaging data sets. This workflow enables precise region-specific comparisons and enriches the anatomical context with robust molecular information.",
  "abstract_shown": true,
  "affiliations": [
    "The Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University, Maastricht6229 ER, The Netherlands.",
    "TissueVision 150 California St STE 303, Newton, MA02458, The United States.",
    "Institute of Molecular Immunology, School of Medicine and Health, Technical University of Munich (TUM), Munich81675, Germany."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ruben Jacobs",
      "orcid": "0009-0002-5933-936X"
    },
    {
      "affs": [
        1
      ],
      "name": "Ritesh Chidambaram",
      "orcid": "0009-0001-1797-1191"
    },
    {
      "affs": [
        2
      ],
      "name": "Vincent Steinbacher"
    },
    {
      "affs": [
        1
      ],
      "name": "Chenwei Wei"
    },
    {
      "affs": [
        1
      ],
      "name": "Tim Ragan"
    },
    {
      "affs": [
        0
      ],
      "name": "Michiel Vandenbosch",
      "orcid": "0000-0002-0427-416X"
    },
    {
      "affs": [
        0
      ],
      "name": "Ron M A Heeren",
      "orcid": "0000-0002-6533-7179"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9v8det8237bbe.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.analchem.6c03106",
  "fulltext_url": "https://europepmc.org/articles/PMC13647983",
  "id": "9v8det8237bbe",
  "issns": [
    "0003-2700",
    "1520-6882"
  ],
  "landing_url": "https://europepmc.org/article/MED/42915698",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Mass Spectrometry Techniques and Applications"
    ]
  ],
  "openalex": "W7214019458",
  "pdf_url": "https://europepmc.org/articles/PMC13647983?pdf=render",
  "pmcid": "PMC13647983",
  "pmid": "42915698",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9v8det8237bbe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42915698",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42915698"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Mass Spectrometry Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Enabling High-Throughput Tissue Sectioning for Robust Lipid Mass Spectrometry Imaging.",
  "title_text": "Enabling High-Throughput Tissue Sectioning for Robust Lipid Mass Spectrometry Imaging.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/9v8det8237bbe",
  "venue": "Analytical chemistry",
  "versions": []
}