{
  "abstract": "Photoactivatable fluorophores are indispensable tools for super-resolution microscopy, but their performance is often hindered by the need for caging group chemistry, ultraviolet activation, interfering triplet-state photochemistry, and most importantly resulting in a single emissive scaffold. Here, we report a hydroacridine-based fluorophore that addresses the often encountered limitations by providing caging-group-free activation using visible light. A heteroatom-engineering strategy enables rapid singlet-state proton-coupled electron transfer, converting a blue-emissive precursor into two highly fluorescent acridinium states with distinct green (ΦF ≈ 0.80) and red (ΦF ≈ 0.78) emission by a two-step photoconversion using blue and green light sequentially. The process operates efficiently under physiological conditions without involving triplet biradical intermediates, enhancing compatibility with live-cell imaging. The fluorophore architecture further supports organelle-targeted imaging and photoinduced intracellular redistribution, enabling multicolour visualization from a single molecular scaffold, serving both spatial and temporal control over the photoactivation. These findings provide new strategies for heteroatom-controlled visible-light photoactivation and fluorophore design, providing a new platform for next-generation super-resolution imaging.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Saugat Mondal",
      "orcid": "0000-0002-5571-2465"
    },
    {
      "name": "Tapas Bera"
    },
    {
      "name": "Debjit Maity"
    },
    {
      "name": "Sayan Paul"
    },
    {
      "name": "Swapnamoy Sahoo"
    },
    {
      "name": "Bhaskar Rao Pinninti"
    },
    {
      "name": "Mahitosh Mandal"
    },
    {
      "name": "Hannes A. Houck"
    },
    {
      "name": "N. D. Pradeep Singh"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9vnd07z5pgy6q.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009929/v1",
  "fulltext_url": null,
  "id": "9vnd07z5pgy6q",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009929/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7220361257",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15009929/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-05",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/9vnd07z5pgy6q.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:22Z",
      "native_id": "10.26434/chemrxiv.15009929",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009929/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Wavelength-Sequential Generation of Multi-Emissive Fluorophores for Site-Selective Fluorescence Nanoscopy",
  "title_text": "Wavelength-Sequential Generation of Multi-Emissive Fluorophores for Site-Selective Fluorescence Nanoscopy",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:22Z",
  "url": "https://research.draconitic.com/paper/9vnd07z5pgy6q",
  "venue": "ChemRxiv",
  "versions": []
}