{
  "abstract": "Heat stress (HS) poses a significant threat to dairy production by reducing milk yield and elevating the incidence of mastitis. Concurrently, mastitis caused by Klebsiella pneumoniae (K. pneumoniae) occurs more frequently during the summer months, further exacerbating udder health challenges. Our study demonstrates that combined HS and K. pneumoniae exposure significantly exacerbates cellular damage of bovine mammary epithelial cells (BMECs). Mechanistically, HS potentiates K. pneumoniae-induced NF-κB pathway activation and reactive oxygen species (ROS) accumulation, leading to upregulated pro-inflammatory cytokines, chemokines, and antimicrobial peptides. Simultaneously, HS disrupts tight junction integrity, downregulating ZO-1, Occludin, and Claudins, and compromises the epithelial barrier. Critically, HS further suppresses lipid accumulation and the expression of milk fat synthesis-related genes and proteins in K. pneumoniae-challenged BMECs. This metabolic suppression is linked to the downregulation of key lipogenic regulators (SREBP1, PPAR-γ) and enzymes (FASN, ACC), alongside upregulated fatty acid oxidation (CPT1A). These findings elucidate how HS aggravates K. pneumoniae-induced mastitis by amplifying inflammation, disrupting barrier function, and inhibiting lactation, providing new insights into summer mastitis pathogenesis.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China",
    "Pancreatic Center, Department of Gastroenterology, Yangzhou Key Laboratory of Pancreatic Disease, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China",
    "Key Laboratory of Animal Schistosomiasis of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China",
    "Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jia Guo"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhihao Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Haoran Zhong"
    },
    {
      "affs": [
        0
      ],
      "name": "Pengfei Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Kangjun Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Long Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Luying Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Junsheng Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Jianji Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Guoqiang Zhu"
    },
    {
      "affs": [
        3
      ],
      "name": "Ran Wang"
    },
    {
      "affs": [
        3
      ],
      "name": "Lili Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xia Meng"
    },
    {
      "affs": [
        0
      ],
      "name": "Heng Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9vyqrpgayb0yw.bib",
  "cited_by": 0,
  "doi": "10.1080/01652176.2026.2747449",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13726294/",
  "id": "9vyqrpgayb0yw",
  "issns": [
    "0165-2176",
    "1875-5941"
  ],
  "landing_url": "https://doi.org/10.1080/01652176.2026.2747449",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1103|Effects of Environmental Stressors on Livestock"
    ]
  ],
  "openalex": "W7224864882",
  "pdf_url": "https://www.tandfonline.com/doi/pdf/10.1080/01652176.2026.2747449?needAccess=true",
  "pmcid": "PMC13726294",
  "pmid": "42962078",
  "published": "2026-10-30",
  "publisher": "Taylor & Francis",
  "ris": "https://research.draconitic.com/paper/9vyqrpgayb0yw.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:22:40Z",
      "native_id": "PMC13726294",
      "source": "pmc",
      "url": "https://doi.org/10.1080/01652176.2026.2747449"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Effects of Environmental Stressors on Livestock",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Heat stress enhances Klebsiella pneumoniae-induced cellular injury and impairs milk fat synthesis in bovine mammary epithelial cells",
  "title_text": "Heat stress enhances Klebsiella pneumoniae-induced cellular injury and impairs milk fat synthesis in bovine mammary epithelial cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:22:17Z",
  "url": "https://research.draconitic.com/paper/9vyqrpgayb0yw",
  "venue": "The Veterinary Quarterly",
  "versions": []
}