{
  "abstract": "Here, we present an enzyme-free workflow for isolating murine adult neural stem cells (aNSCs) using tissue grinder- and dissociator-based mechanical dissociation (TIGR-MD). We describe dissection of the dentate gyrus and subventricular zone, mechanical tissue dissociation, cell recovery, and establishment of primary neurosphere cultures. We further provide procedures for neurosphere maintenance, passaging, and validation. TIGR-MD avoids enzymatic exposure during initial tissue dissociation and supports standardized establishment of primary murine aNSC cultures.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurology and Interdisciplinary Neuro-oncology, Hertie Institute for Clinical Brain Research, University Hospital Tübingen, Tübingen, Germany",
    "M3 Research Center for Malignome, Metabolome and Microbiome, University Hospital Tübingen, Tübingen, Germany",
    "Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany",
    "Cluster of Excellence iFIT (EXC 2180) ‘Image-Guided and Functionally Instructed Tumor Therapies’, University of Tübingen, Tübingen, Germany",
    "Faculty of Pharmacy, University of Castilla-La Mancha, Albacete, Spain",
    "Instituto Regional de Investigación Científica Aplicada (IRICA), University of Castilla-La Mancha, Ciudad Real, Spain"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Vanessa Arnold"
    },
    {
      "affs": [
        0,
        1,
        2,
        4,
        5
      ],
      "name": "Anna Wolfram"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Anastassiya Moskalchuk"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Vijayasarathy Sampath-Kumar"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Abhirami Sunil"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Sogand Ahari"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Lisa Sevenich"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9wrqxnfpsbam8.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104908",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13698050/",
  "id": "9wrqxnfpsbam8",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104908",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "2806|Neurogenesis and neuroplasticity mechanisms"
    ]
  ],
  "openalex": "W7223041170",
  "pdf_url": "https://europepmc.org/articles/PMC13698050?pdf=render",
  "pmcid": "PMC13698050",
  "pmid": "42900586",
  "published": "2026-10-20",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/9wrqxnfpsbam8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "PMC13698050",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104908"
    },
    {
      "first_seen": "2026-10-30T12:19:32Z",
      "native_id": "MED:42900586",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42900586"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2806|Neurogenesis and neuroplasticity mechanisms",
      "subfield": "neuroscience"
    }
  ],
  "title": "Protocol for isolation and culture establishment of adult murine neural stem cells using tissue grinder and dissociator-based mechanical dissociation",
  "title_text": "Protocol for isolation and culture establishment of adult murine neural stem cells using tissue grinder and dissociator-based mechanical dissociation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:59Z",
  "url": "https://research.draconitic.com/paper/9wrqxnfpsbam8",
  "venue": "STAR Protocols",
  "versions": []
}