{
  "abstract": "NF-κB transcription factor (TF) activation is heterogeneous across identically stimulated cells, shaping innate immune responses. Here, we present a protocol to quantify this heterogeneity in live Drosophila melanogaster S2∗ cells using dual reporters of NF-κB (Relish) subcellular localization and downstream transcription. We describe steps for reporter cell line generation, immune stimulation and time-lapse confocal imaging, and machine-learning-based single-cell analysis to classify signaling behaviors and predict transcriptional responses.\n\nFor complete details on the use and execution of this protocol, please refer to Nawar et al.1",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA",
    "Biological Design Center, Boston University, Boston, MA 02215, USA",
    "Department of Biology, Boston University, Boston, MA 02215, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Noshin Nawar"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Emma Rits"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Zeba Wunderlich"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9y3c8cwwrw56x.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104902",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13717862/",
  "id": "9y3c8cwwrw56x",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104902",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1306|NF-κB Signaling Pathways"
    ]
  ],
  "openalex": "W7223022217",
  "pdf_url": null,
  "pmcid": "PMC13717862",
  "pmid": "42905510",
  "published": "2026-10-20",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/9y3c8cwwrw56x.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:22:05Z",
      "native_id": "PMC13717862",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104902"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|NF-κB Signaling Pathways",
      "subfield": "oncology"
    }
  ],
  "title": "Protocol for live-cell imaging and analysis of NF-κB localization and target gene transcription in Drosophila S2∗ cells",
  "title_text": "Protocol for live-cell imaging and analysis of NF-κB localization and target gene transcription in Drosophila S2∗ cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:05Z",
  "url": "https://research.draconitic.com/paper/9y3c8cwwrw56x",
  "venue": "STAR Protocols",
  "versions": []
}