{
  "abstract": "Sister chromatid cohesion depends on ESCO2-mediated acetylation of the cohesin subunit SMC3, yet how this modification is established and maintained across the genome remains poorly understood. Here, we examined the chromatin-binding dynamics of ESCO2 and its enzymatic activity using quantitative ChIP-seq of human cells synchronously progressing through S phase. ESCO2 bound broadly along chromosomes without forming discrete peaks and preferentially localized to transcriptionally inactive regions, but was not confined to H3K9me3- or H3K27me3-enriched heterochromatin. Consistent with this binding pattern, ESCO2-dependent SMC3 acetylation showed a similarly broad distribution in these regions. In contrast, ESCO1, a paralog of ESCO2, primarily mediated SMC3 acetylation at cohesin peak sites. Notably, most of the broadly distributed, ESCO2-dependent SMC3 acetylation generated during S phase was progressively lost from chromatin after replication, whereas the fraction that persisted into late G2 accumulated in domains enriched for the repressive H3K27me3 modification. Our work identifies selective post-replicative retention of acetylated cohesin as a previously unappreciated step in shaping the genome-wide architecture of sister chromatid cohesion.",
  "abstract_shown": true,
  "affiliations": [
    "Institute for Quantitative Biosciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032 ,",
    "Faculty of Core Research, Natural Sciences Division, Ochanomizu University , 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610 ,",
    "Institute of Medical Genetics, Tokyo Women’s Medical University , 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666 ,",
    "Department of Cell and Molecular Biology, Karolinska Institutet, Biomedicum , Tomtebodavägen 16, 171 77 Stockholm ,",
    "Division of Biological Sciences, Graduate School of Science, Kyoto University , Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502 ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Mai I Shimizu"
    },
    {
      "affs": [
        0
      ],
      "name": "Masashige Bando"
    },
    {
      "affs": [
        0
      ],
      "name": "Atsunori Yoshimura"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Toyonori Sakata"
    },
    {
      "affs": [
        4
      ],
      "name": "Tomoko Nishiyama"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Katsuhiko Shirahige",
      "orcid": "0000-0002-7862-1144"
    },
    {
      "affs": [
        0
      ],
      "name": "Takashi Sutani",
      "orcid": "0000-0002-9959-9513"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9zj4mczg8p0zd.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag1061",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13722876/?tool=EBI",
  "id": "9zj4mczg8p0zd",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1061/9005799",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "AcademicSubjects/SCI00010"
    ],
    [
      "heading",
      "Genome Integrity, Repair and Replication"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://academic.oup.com/nar/article-pdf/54/20/gkag1061/72640320/gkag1061.pdf",
  "pmcid": "PMC13722876",
  "pmid": "42965290",
  "published": "2026-10-31",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/9zj4mczg8p0zd.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:18Z",
      "native_id": "10.1093/nar/gkag1061",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1061/9005799"
    },
    {
      "first_seen": "2026-11-02T03:21:51Z",
      "native_id": "PMC:PMC13722876",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13722876"
    },
    {
      "first_seen": "2026-11-04T12:21:37Z",
      "native_id": "PMC13722876",
      "source": "pmc",
      "url": "https://doi.org/10.1093/nar/gkag1061"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:0305-1048",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Region-specific generation and retention of acetylated cohesin shape the genome-wide pattern of sister chromatid cohesion",
  "title_text": "Region-specific generation and retention of acetylated cohesin shape the genome-wide pattern of sister chromatid cohesion",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:21:37Z",
  "url": "https://research.draconitic.com/paper/9zj4mczg8p0zd",
  "venue": "Nucleic Acids Research",
  "versions": []
}