{
  "abstract": "Idiopathic pulmonary fibrosis (IPF) is characterized by persistent fibroblast activation and excessive extracellular matrix deposition. However, transcriptional heterogeneity associated with translation- and RNA-binding protein (RBP)-related processes within IPF fibroblasts remains incompletely characterized. Here, we analyzed two independent human IPF single-cell RNA-sequencing cohorts to identify fibroblast states that differed in Translation/RBP-related transcriptional programs while sharing high fibrotic output. Donor-paired pseudobulk analysis in GSE136831 identified a transcriptional signature distinguishing these states, and genes showing consistent directional changes in the independent GSE135893 cohort were retained to derive a cross-cohort signature. Reverse-connectivity analysis across CLUE, L1000CDS2, and iLINCS was then used to prioritize compounds predicted to transcriptionally oppose this signature. Candidate compounds were subsequently evaluated in transforming growth factor-β1 (TGF-β1)-stimulated human fetal lung fibroblasts (HFL-1), with cell viability, α-smooth muscle actin (α-SMA), and collagen I used for initial phenotypic assessment. PP-1, PK-11195, dabrafenib, GSK-1059615, and dasatinib reduced both profibrotic markers within the tested concentration ranges, identifying them as candidates for further investigation. Together, these findings define a cross-cohort Translation/RBP-related fibroblast signature in IPF and support its use as a transcriptome-guided strategy for prioritizing compounds with antifibrotic phenotypic activity.",
  "abstract_shown": true,
  "affiliations": [
    "Center for Biological Science and Technology, Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Guangdong Zhuhai-Macao Joint Biotech Laboratory, Department of Biology, Faculty of Arts and Sciences, Beijing Normal University, Zhuhai 519087, China",
    "School of Pharmacy, Mudanjiang Medical University, Mudanjiang 157011, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yongkang Qiao",
      "orcid": "0000-0002-1052-6722"
    },
    {
      "affs": [
        0
      ],
      "name": "Chaoyue Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanhong Han"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xinxin Tang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhen Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Chuang Ge"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a3en14x7vb0cn.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219736",
  "fulltext_url": null,
  "id": "a3en14x7vb0cn",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9736",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/a3en14x7vb0cn.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:54Z",
      "native_id": "10.3390/ijms27219736",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9736"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Single-Cell Transcriptomic Analysis Identifies a Translation/RBP-Related Fibroblast Signature for Drug Repurposing in Idiopathic Pulmonary Fibrosis",
  "title_text": "Single-Cell Transcriptomic Analysis Identifies a Translation/RBP-Related Fibroblast Signature for Drug Repurposing in Idiopathic Pulmonary Fibrosis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T00:20:52Z",
  "url": "https://research.draconitic.com/paper/a3en14x7vb0cn",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}