{ "abstract": "This study aimed to establish epidemiological cutoff values (ECVs) for amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, and isavuconazole against Talaromyces marneffei (T. marneffei) using the CLSI broth microdilution method. Antifungal susceptibility testing of 207 T. marneffei isolates was performed independently at five laboratories across China according to CLSI M27-A4 guidelines. Minimum inhibitory concentration (MIC) distributions were analyzed using ECOFFinder software (version 2.1), and ECVs were set at the upper MIC limit encompassing 97.5% of the modeled wild-type (WT) population. ECVs were determined as follows: amphotericin B (AMB) 8 µg/mL, voriconazole (VRC) 0.03 µg/mL, itraconazole (ITC) 0.125 µg/mL, posaconazole (POS) 0.06 µg/mL, isavuconazole (ISA) 0.125 µg/mL, and fluconazole (FLC) 8 µg/mL. MIC distributions for all six agents conformed well to the fitted models (normal or log-normal), supporting the validity of ECV determination. No non-WT isolates were detected for amphotericin B, whereas non-WT rates for azoles were 15.5% (voriconazole), 8.45% (itraconazole), 6.37% (posaconazole), 3.0% (fluconazole), and 1.02% (isavuconazole). Among voriconazole non-WT isolates, all were from patients with prior voriconazole exposure. This study provides the first standardized ECVs for T. marneffei against six key antifungal agents. The data establish a reference framework for antifungal susceptibility testing and resistance surveillance and highlight the need for continued monitoring of azole susceptibility in this pathogen.", "abstract_shown": true, "affiliations": [ "Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China", "Guangxi Scientific and Technological Innovation Cooperation Base of Mycosis Prevention and Control, Nanning, China", "Department of Dermatology and Venerology, Peking University First Hospital, Beijing, China", "Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China", "Department of Medical Mycology, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China", "Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China", "National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China", "The First Affiliated Hospital of Guilin Medical University, Guilin, China", "The Fourth People’s Hospital of Nanning, Nanning, China", "Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang, China", "Dermatology Hospital of Southern Medical University, Guangzhou, China", "Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China", "Centre for Infectious Diseases Research, Diagnostics and Laboratory Surveillance, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands", "Department of Medical Microbiology and Radboudumc - CWZ Centre of Expertise for Mycology, Radboud University Medical Centre, Nijmegen, the Netherlands" ], "article_type": "research article", "arxiv": null, "author_total": 21, "authors": [ { "affs": [ 0, 1 ], "name": "Kai-su Pan", "orcid": "0000-0002-8328-3776" }, { "affs": [ 2 ], "name": "Ying-gai Song", "orcid": "0000-0002-1644-6942" }, { "affs": [ 3 ], "name": "Sha Lu" }, { "affs": [ 4 ], "name": "Xiao-fang Li", "orcid": "0000-0002-5148-2922" }, { "affs": [ 5 ], "name": "Huai-qiu Huang" }, { "affs": [ 6 ], "name": "Jie Gong" }, { "affs": [ 2 ], "name": "Ruo-yu Li" }, { "affs": [ 4 ], "name": "Hai-lin Zheng", "orcid": "0000-0003-0247-1853" }, { "affs": [ 5 ], "name": "Miao-rong Zhan" }, { "affs": [ 6 ], "name": "Shu Zhang" }, { "affs": [ 0, 7 ], "name": "Xin-yu Zhou" }, { "affs": [ 6 ], "name": "Yuan-yuan Geng" }, { "affs": [ 0, 1 ], "name": "Si-wen Jiang" }, { "affs": [ 0, 1 ], "name": "Sheng-xiu Fang" }, { "affs": [ 8, 9 ], "name": "Yanqing Zheng", "orcid": "0000-0002-7809-5527" }, { "affs": [ 0, 9 ], "name": "Dong-yan Zheng" }, { "affs": [ 0, 9 ], "name": "Weixuan Wu" }, { "affs": [ 3, 10 ], "name": "Li-yan Xi", "orcid": "0000-0003-1504-0147" }, { "affs": [ 11 ], "name": "He-ping Xu", "orcid": "0000-0002-0552-7122" }, { "affs": [ 12, 13 ], "name": "Paul E. Verweij", "orcid": "0000-0002-8600-9860" }, { "affs": [ 0, 1 ], "name": "Cun-wei Cao", "orcid": "0000-0002-6279-0322" } ], "bibtex": "https://research.draconitic.com/paper/a3z0yy4jwgcst.bib", "cited_by": 0, "doi": "10.1128/aac.00650-26", "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13712858/", "id": "a3z0yy4jwgcst", "issns": [ "0066-4804", "1098-6596" ], "landing_url": "https://doi.org/10.1128/aac.00650-26", "language": "en", "licence": "CC-BY-4.0", "licence_label": "CC BY 4.0", "licence_source": "article", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "heading", "Susceptibility" ], [ "openalex", "2725|Antifungal resistance and susceptibility" ] ], "openalex": "W7172548670", "pdf_url": "https://journals.asm.org/doi/pdf/10.1128/aac.00650-26?download=true", "pmcid": "PMC13712858", "pmid": "42554647", "published": "2026-08-05", "publisher": "American Society for Microbiology (ASM)", "ris": "https://research.draconitic.com/paper/a3z0yy4jwgcst.ris", "sources": [ { "first_seen": "2026-10-31T12:23:21Z", "native_id": "PMC13712858", "source": "pmc", "url": "https://doi.org/10.1128/aac.00650-26" } ], "subjects": [ { "field": "medicine", "origin": "openalex:2725|Antifungal resistance and susceptibility", "subfield": "infectious-disease" } ], "title": "Establishment of epidemiological cutoff values for yeast-phase Talaromyces marneffei against six antifungal agents: a multicenter study in China", "title_text": "Establishment of epidemiological cutoff values for yeast-phase Talaromyces marneffei against six antifungal agents: a multicenter study in China", "type": "peer-reviewed", "updated_at": "2026-10-31T12:23:21Z", "url": "https://research.draconitic.com/paper/a3z0yy4jwgcst", "venue": "Antimicrobial Agents and Chemotherapy", "versions": [] }