{
  "abstract": "Electron diffraction (MicroED/3DED) enables atomic structure determination from nano- or microcrystals, including dry crystalline powders or mixtures. However, radiation damage and instrument vacuum can limit the interrogation of molecular crystals in solution at room temperature. We now present a strategy for room-temperature MicroED of organic molecular microcrystals suspended in solution, using simple liquid cells: grid sandwiches comprised of two continuous carbon grids that when pressed retain a thin liquid layer between them. In this setting, we find that some room-temperature, hydrated microcrystals are particularly prone to electron beam radiation damage, requiring fast, low dose data collection schemes, direct electron detectors, and multi-crystal data collection. These approaches minimize damage of crystals in liquid cells and facilitate ab initio structures at sub-Ångstrom resolution. This tactic enables study of molecular conformations and crystalline phases that may only be accessible in a room-temperature solvated state.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), Los Angeles, California 90095, United States",
    "UCLA-DOE Institute for Genomics and Proteomics; STROBE, NSF Science and Technology Center, University of California, Los Angeles (UCLA), Los Angeles, California 90095, United States"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Niko W. Vlahakis"
    },
    {
      "affs": [
        0
      ],
      "name": "Krzysztof Konieczny",
      "orcid": "0000-0003-4618-5154"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Cameron W. Flowers"
    },
    {
      "affs": [
        0
      ],
      "name": "Miguel A. Garcia-Garibay"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jose A. Rodriguez",
      "orcid": "0000-0002-0248-4964"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a47qsqadgjp3b.bib",
  "cited_by": 1,
  "doi": "10.1021/acscentsci.6c00603",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13720921/?tool=EBI",
  "id": "a47qsqadgjp3b",
  "issns": [
    "2374-7943",
    "2374-7951"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13720921",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1315|Advanced Electron Microscopy Techniques and Applications"
    ]
  ],
  "openalex": "W7214036072",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13720921/pdf/?tool=EBI",
  "pmcid": "PMC13720921",
  "pmid": "42964980",
  "published": "2026-09-22",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/a47qsqadgjp3b.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:36Z",
      "native_id": "PMC:PMC13720921",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13720921"
    },
    {
      "first_seen": "2026-11-03T12:21:43Z",
      "native_id": "PMC13720921",
      "source": "pmc",
      "url": "https://doi.org/10.1021/acscentsci.6c00603"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1315|Advanced Electron Microscopy Techniques and Applications",
      "subfield": "structural-biology"
    }
  ],
  "title": "Room Temperature Structures of Solvated Organic Molecules Enabled by Liquid Cell Electron Diffraction",
  "title_text": "Room Temperature Structures of Solvated Organic Molecules Enabled by Liquid Cell Electron Diffraction",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:43Z",
  "url": "https://research.draconitic.com/paper/a47qsqadgjp3b",
  "venue": "ACS central science",
  "versions": []
}