{
  "abstract": "Background and Objectives: Enterococcus faecium is a major nosocomial pathogen frequently associated with catheter-re- lated infections and biofilm-mediated antimicrobial resistance. This study aimed to investigate the genomic determinants and structural characteristics underlying biofilm formation in a clinical E. faecium isolate recovered from a catheter-associated urinary tract infection (CAUTI). Materials and Methods: A clinical isolate, E. faecium MFKCC-BF.UR33, was subjected to whole-genome sequencing and comprehensive genome annotation to identify biofilm-associated and antimicrobial resistance genes. Phylogenetic analysis was performed to determine its relatedness to reference clinical strains. Structural modeling of selected biofilm-associated proteins was conducted using AlphaFold2 to assess their three-dimensional conformations and potential as therapeutic targets. Results: Whole-genome sequencing revealed a 3.32 Mb genome with a G+C content of 37.35%, comprising 2,321 predicted coding sequences and no detectable plasmids. Genome annotation identified several biofilm-associated genes, including sgrA, acm, fss3, scm, and efbA, as well as antimicrobial resistance genes (antA/B, tetM, tetL, efmA, and liaFSR). Phylogeneticanalysis placed the isolate within the E. faecium clade, closely related to other clinical reference strains. Structural modeling demonstrated high-confidence 3D structures for Fss3 and EfbA proteins. Conclusion: The integration of genomic and structural analyses provides insights into the genetic and structural basis of biofilm formation in E. faecium and establishes a foundation for future functional and structure-based investigations.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Ayu Rahayu"
    },
    {
      "name": "Sayyidah Auliany Aminy"
    },
    {
      "name": "Dian Asti Umaroh"
    },
    {
      "name": "Niar Gusnaniar"
    },
    {
      "name": "Akhirta Atikana"
    },
    {
      "name": "Puspita Lisdiyanti"
    },
    {
      "name": "Estelle Perraud"
    },
    {
      "name": "Christine Imbert"
    },
    {
      "name": "Titik Nuryastuti"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a4fq7bqvnjr6p.bib",
  "cited_by": 0,
  "doi": "10.18502/ijm.v18i5.22866",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13738240/",
  "id": "a4fq7bqvnjr6p",
  "issns": [
    "2008-3289",
    "2008-4447"
  ],
  "landing_url": "https://hal.science/hal-05788268v1",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1312|Bacterial biofilms and quorum sensing"
    ]
  ],
  "openalex": "W7219822923",
  "pdf_url": "https://hal.science/hal-05788268/document",
  "pmcid": "PMC13738240",
  "pmid": "42992164",
  "published": "2026-10-09",
  "publisher": "Tehran University of Medical Sciences",
  "ris": "https://research.draconitic.com/paper/a4fq7bqvnjr6p.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:52Z",
      "native_id": "hal-05788268",
      "source": "hal",
      "url": "https://hal.science/hal-05788268v1"
    },
    {
      "first_seen": "2026-11-09T12:23:15Z",
      "native_id": "PMC13738240",
      "source": "pmc",
      "url": "https://doi.org/10.18502/ijm.v18i5.22866"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
    },
    {
      "field": "biology",
      "origin": "hal:1.sdv.mp",
      "subfield": "microbiology"
    },
    {
      "field": "medicine",
      "origin": "hal:2.sdv.mhep.mi",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Genomic insight into Enterococcus faecium biofilm formation isolated from catheter associated urinary tract infection",
  "title_text": "Genomic insight into Enterococcus faecium biofilm formation isolated from catheter associated urinary tract infection",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:23:15Z",
  "url": "https://research.draconitic.com/paper/a4fq7bqvnjr6p",
  "venue": "Iranian Journal of Microbiology",
  "versions": []
}