{
  "abstract": "Peanut (Arachis hypogaea) is an allotetraploid legume crop of agricultural and economic importance worldwide. Despite advances in peanut genomics, the integration of genetic diversity with multi‐omics landscapes remains limited, and a comprehensive understanding of subgenome asymmetry is still lacking. Here, we present a high‐quality genome assembly of an elite peanut cultivar Zhonghua 5 (ZH05; 2,622.85 Mb with 73,718 predicted genes) and construct a species‐specific pan‐genome based on 1,286 accessions, uncovering 849.82 Mb of non‐reference sequences and 18,484 novel genes. Over 23 million genetic variants and extensive gene PAVs substantially expand the landscape of genetic diversity in peanut. Integrative multi‐omics (transcriptome, DNA methylation, chromatin accessibility, and three‐dimensional genome architecture), combined with population genomics analyses, reveal pronounced and multi‐layered subgenome asymmetry. Specifically, SubA shows higher chromatin accessibility and overall gene expression, whereas SubB displays more transposable elements, elevated DNA methylation, more genes under selection during domestication, and stronger three‐dimensional chromatin interactions with more stable topologically associating domains. Furthermore, genetic dissection using a recombinant inbred line population identified several major stable QTLs for seed size, whose pyramiding contributes to ZH05's superior yield performance. Collectively, this study establishes an integrated genomic framework for peanut, illuminates asymmetric subgenome evolution across sequence, epigenetic, and chromatin architecture, and provides valuable resources for accelerating molecular breeding and crop improvement.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs Oil Crops Research Institute of Chinese Academy of Agricultural Sciences (OCRI‐CAAS) Wuhan 430062 China",
    "National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 China",
    "Biobreeding Institute Xianghu Laboratory Hangzhou 311231 China",
    "Zhanjiang Academy of Agricultural Sciences Zhanjiang 524000 China",
    "Nanchong Academy of Agricultural Sciences Nanchong 637000 China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 20,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Taihua Yang",
      "orcid": "0000-0003-1484-1989"
    },
    {
      "affs": [
        0
      ],
      "name": "Nian Liu",
      "orcid": "0000-0003-1034-028X"
    },
    {
      "affs": [
        0
      ],
      "name": "Li Huang",
      "orcid": "0009-0002-0237-5247"
    },
    {
      "affs": [
        0
      ],
      "name": "Jianbin Guo",
      "orcid": "0009-0008-2668-5526"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiaomeng Xue",
      "orcid": "0009-0001-8059-7315"
    },
    {
      "affs": [
        0
      ],
      "name": "Mingjun Wang",
      "orcid": "0009-0003-5636-9747"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaohan Zhang",
      "orcid": "0009-0008-4728-5951"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Chengtao Quan",
      "orcid": "0009-0000-2760-3539"
    },
    {
      "affs": [
        3
      ],
      "name": "Enyou Feng",
      "orcid": "0009-0002-8999-0735"
    },
    {
      "affs": [
        3
      ],
      "name": "Jiazhuang Tan",
      "orcid": "0009-0009-9093-3130"
    },
    {
      "affs": [
        4
      ],
      "name": "Zhenzhen Zhang",
      "orcid": "0009-0007-0381-5853"
    },
    {
      "affs": [
        4
      ],
      "name": "Yu You",
      "orcid": "0009-0006-0496-8336"
    },
    {
      "affs": [
        4
      ],
      "name": "Youlin Xia",
      "orcid": "0009-0005-6999-1863"
    },
    {
      "affs": [
        0
      ],
      "name": "Dongxin Huai",
      "orcid": "0000-0003-0529-2199"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuning Chen",
      "orcid": "0000-0002-0828-9414"
    },
    {
      "affs": [
        0
      ],
      "name": "Liying Yan",
      "orcid": "0000-0002-4355-683X"
    },
    {
      "affs": [
        1
      ],
      "name": "Kede Liu",
      "orcid": "0000-0002-1395-2049"
    },
    {
      "affs": [
        0
      ],
      "name": "Huifang Jiang",
      "orcid": "0000-0003-4087-205X"
    },
    {
      "affs": [
        0
      ],
      "name": "Yong Lei",
      "orcid": "0000-0002-3738-4197"
    },
    {
      "affs": [
        0
      ],
      "name": "Boshou Liao",
      "orcid": "0000-0002-6960-8175"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a5kt7zhxdz256.bib",
  "cited_by": 0,
  "doi": "10.1111/jipb.70308",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13725605/",
  "id": "a5kt7zhxdz256",
  "issns": [
    "1672-9072",
    "1744-7909"
  ],
  "landing_url": "https://doi.org/10.1111/jipb.70308",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Functional Omics and Systems Biology"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1110|Peanut Plant Research Studies"
    ]
  ],
  "openalex": "W7163144494",
  "pdf_url": null,
  "pmcid": "PMC13725605",
  "pmid": "42226345",
  "published": "2026-06-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/a5kt7zhxdz256.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:23:57Z",
      "native_id": "PMC13725605",
      "source": "pmc",
      "url": "https://doi.org/10.1111/jipb.70308"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Peanut Plant Research Studies",
      "subfield": "plant-biology"
    }
  ],
  "title": "High‐quality genome of elite peanut cultivar ZH05 reveals subgenome asymmetry, pan‐genome diversity, and breeding insights",
  "title_text": "High‐quality genome of elite peanut cultivar ZH05 reveals subgenome asymmetry, pan‐genome diversity, and breeding insights",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:48Z",
  "url": "https://research.draconitic.com/paper/a5kt7zhxdz256",
  "venue": "Journal of Integrative Plant Biology",
  "versions": []
}