{
  "abstract": "Polyethylene terephthalate (PET) is a major contributor to plastic waste. Enzymatic PET degradation offers a sustainable recycling approach, but screening for effective enzymes remains challenging. Herein, we established an experimental plate-clearing assay using Vibrio natriegens, exploiting its protein secretion system to rapidly identify catalytic activity without time-consuming downstream processing. To validate the assay’s robustness, we tested variants of Fusarium solanipisi cutinase (FsC) and found that one variant, T45P, exhibited 3-fold higher PET-film degradation yield than the wild type and favored the production of terephthalic acid. As a further test case, we screened an error-prone polymerase chain reaction (PCR) library of FsC using the assay, obtaining a 25% hit rate after screening 150 colonies. This demonstrates the scalability of the method to large enzyme libraries.",
  "abstract_shown": true,
  "affiliations": [
    "NTNU , , Sem Sælands vei 6/8 , ,",
    "Aalborg University , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Susanne H. Troøyen"
    },
    {
      "affs": [
        0
      ],
      "name": "Ingrid M. Bolstad"
    },
    {
      "affs": [
        0
      ],
      "name": "Nora Drøivoldsmo"
    },
    {
      "affs": [
        0
      ],
      "name": "Davide Luciano"
    },
    {
      "affs": [
        1
      ],
      "name": "Evamaria I. Petersen"
    },
    {
      "affs": [
        0
      ],
      "name": "Gaston Courtade",
      "orcid": "0000-0002-1644-3223"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a6ewqb0vxdsqv.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c05931",
  "fulltext_url": null,
  "id": "a6ewqb0vxdsqv",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c05931/5464832/Development-of-a-Vibrio-natriegens-Based-Plate",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c05931",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/a6ewqb0vxdsqv.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:20:52Z",
      "native_id": "10.1021/acsomega.6c05931",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c05931/5464832/Development-of-a-Vibrio-natriegens-Based-Plate"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Development of a Vibrio natriegens -Based Plate-Clearing Assay for Rapid Screening of PET-Hydrolyzing Enzymes",
  "title_text": "Development of a Vibrio natriegens -Based Plate-Clearing Assay for Rapid Screening of PET-Hydrolyzing Enzymes",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T00:21:06Z",
  "url": "https://research.draconitic.com/paper/a6ewqb0vxdsqv",
  "venue": "ACS Omega",
  "versions": []
}