{
  "abstract": "Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.",
  "abstract_shown": true,
  "affiliations": [
    "Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy. moises.disante@unipv.it",
    "Synthetic Physiology Laboratory, University of Pavia, Italy",
    "Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy",
    "Human Technopole, Milan, Italy",
    "Molecular Biotechnology Center \"Guido Tarone\", University of Torino, Torino, Italy",
    "Molecular Biotechnology Center Guido Tarone, University of Torino, Italy",
    "Centro Grandi Strumenti, University of Pavia, Pavia, Italy",
    "Department of Molecular Medicine, University of Pavia, Pavia, Italy",
    "Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy. francesco.pasqualini@unipv.it"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Moises Di Sante",
      "orcid": "0000-0002-4174-1257"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Melissa Pezzotti",
      "orcid": "0009-0008-4737-1825"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Julius Zimmermann",
      "orcid": "0000-0003-1486-0426"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Alessandro Enrico",
      "orcid": "0000-0002-8821-6759"
    },
    {
      "affs": [
        3
      ],
      "name": "J.R. Deschamps",
      "orcid": "0000-0001-8462-2883"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Elisa Balmas",
      "orcid": "0000-0002-4600-6809"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Silvia Becca",
      "orcid": "0009-0009-4918-1133"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Eloisa Torchia",
      "orcid": "0000-0001-6401-9438"
    },
    {
      "affs": [
        2,
        5
      ],
      "name": "Giulia Ballio"
    },
    {
      "affs": [
        6,
        3
      ],
      "name": "Samantha Solito",
      "orcid": "0000-0003-2544-1673"
    },
    {
      "affs": [
        7,
        1
      ],
      "name": "Martina Sarchi",
      "orcid": "0000-0002-1557-6577"
    },
    {
      "affs": [
        2
      ],
      "name": "Alessandro Reali",
      "orcid": "0000-0002-0639-7067"
    },
    {
      "affs": [
        4
      ],
      "name": "Alessandro Bertero",
      "orcid": "0000-0002-4919-9087"
    },
    {
      "affs": [
        3
      ],
      "name": "Florian Jug",
      "orcid": "0000-0002-8499-5812"
    },
    {
      "affs": [
        8
      ],
      "name": "Francesco S. Pasqualini",
      "orcid": "0000-0001-7038-3121"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a6qae4t0qcpm9.bib",
  "cited_by": 7,
  "doi": "10.1038/s41467-026-76144-9",
  "fulltext_url": null,
  "id": "a6qae4t0qcpm9",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://doi.org/10.1038/s41467-026-76144-9",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
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      "1304|Cell Image Analysis Techniques"
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  "openalex": "W4405682607",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/a6qae4t0qcpm9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:48Z",
      "native_id": "W4405682607",
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      "url": "https://doi.org/10.1038/s41467-026-76144-9"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
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  ],
  "title": "CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies",
  "title_text": "CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T13:20:30Z",
  "url": "https://research.draconitic.com/paper/a6qae4t0qcpm9",
  "venue": "Nature Communications",
  "versions": []
}