{
  "abstract": "Mediator complex subunit 19 (MED19), a component of the Mediator complex, has emerged as a potential regulator of cancer progression, although its downstream transcriptional programs remain poorly understood. Here, we investigated the role of MED19 across 20 human cancer cell lines. An siRNA-mediated knockdown (KD) screen identified A549 lung cancer and MCF-7 breast cancer cells as particularly sensitive to MED19 KD, exhibiting markedly reduced cell viability. Flow cytometric analysis revealed that MED19 KD induced G1-phase arrest, suggesting a role in G1/S-phase progression. RNA sequencing followed by differential expression, Gene Ontology (GO), and chromosomal mapping analyses identified amphiregulin (AREG) and cyclin-dependent kinase regulatory subunit 1B (CKS1B) as candidate downstream effectors in A549 and MCF-7 cells, respectively. Knockdown of AREG or CKS1B inhibited cell proliferation and recapitulated the G1-phase arrest induced by MED19 KD. Moreover, ectopic expression of AREG or CKS1B partially rescued the cell-cycle defects caused by MED19 KD. These findings identify MED19 as a context-dependent regulator of cancer cell proliferation and G1/S-phase progression through distinct downstream transcriptional programs in lung and breast cancer cells.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan",
    "Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan",
    "Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Fukuoka 804-8550, Japan",
    "Radiation Effects Research Foundation, Hiroshima 732-0815, Japan"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sailakshmi Iyer",
      "orcid": "0009-0007-1706-9839"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Naoko Hattori",
      "orcid": "0000-0002-3142-8400"
    },
    {
      "affs": [
        0
      ],
      "name": "Takeya Nakagawa",
      "orcid": "0009-0003-0687-2248"
    },
    {
      "affs": [
        2
      ],
      "name": "Satoshi Fujii",
      "orcid": "0000-0002-3341-8644"
    },
    {
      "affs": [
        3
      ],
      "name": "Tomonori Hayashi"
    },
    {
      "affs": [
        0
      ],
      "name": "Takashi Ito"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a91eqwfgxgmbb.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms272110004",
  "fulltext_url": null,
  "id": "a91eqwfgxgmbb",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/10004",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-08",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/a91eqwfgxgmbb.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:39Z",
      "native_id": "10.3390/ijms272110004",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/10004"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "MED19 Knockdown Induces G1 Cell-Cycle Arrest Through Downregulation of AREG and CKS1B in Lung and Breast Cancer Cells",
  "title_text": "MED19 Knockdown Induces G1 Cell-Cycle Arrest Through Downregulation of AREG and CKS1B in Lung and Breast Cancer Cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:39Z",
  "url": "https://research.draconitic.com/paper/a91eqwfgxgmbb",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}