{
  "abstract": "A stable and active biocatalyst for fructooligosaccharide (FOS) production was synthesized by immobilizing A. oryzae IPT-301 cells on a polyurethane sponge (PS) during submerged cultivation. Comparisons between immobilized and cross-linked immobilized cells revealed similar biochemical properties. The highest cell concentration immobilized in PS (11.89 ± 2.29 g cells g support–1) was obtained after 32 h. Both biocatalysts performed optimally at 50 °C, pH 5.5, and 480.2 g L–1 sucrose, showing a Vmax of 3500 μmol g–1 min–1 and high selectivity for 1-kestose (28.23%). They exhibited a half-life of 300 min at 50 °C, retained 40% activity after 28 days of storage and 50% activity after 12 reaction cycles. FOS productivity reached 116 g L–1 h–1 with a 24% yield. Statistical analysis confirmed that cross-linking did not significantly influence performance. The results show that in situ immobilization on PS is a simple, efficient strategy for producing stable biocatalysts for FOS synthesis.",
  "abstract_shown": true,
  "affiliations": [
    "Federal University of Alfenas , , , ,",
    "University of Minho , , Campus de Gualtar , ,",
    "Institute for Technological Research , , , ,",
    "São Paulo State University , , , ,",
    "Federal University of Tocantins , , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nathália Romani Barbosa"
    },
    {
      "affs": [
        0
      ],
      "name": "Ana Vieira"
    },
    {
      "affs": [
        0
      ],
      "name": "Fábio Aurélyo Silva Ferreira"
    },
    {
      "affs": [
        0
      ],
      "name": "Luara Cristina Alves"
    },
    {
      "affs": [
        1
      ],
      "name": "Sara Silverio"
    },
    {
      "affs": [
        0
      ],
      "name": "Sylma Carvalho Maestrelli"
    },
    {
      "affs": [
        2
      ],
      "name": "Sérgio Fernandes"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Alfredo Eduardo Maiorano"
    },
    {
      "affs": [
        2
      ],
      "name": "Elda Sabino da Silva"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Cristiane Ottoni"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Sergio Villalba Morales"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Rafael Firmani Perna",
      "orcid": "0000-0003-3195-8898"
    },
    {
      "affs": [
        4
      ],
      "name": "Michelle Xavier"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/a9zafpw0k701e.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c07568",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718208/?tool=EBI",
  "id": "a9zafpw0k701e",
  "issns": [
    "2470-1343"
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  "landing_url": "https://pubs.acs.org/acsodf/article/11/42/65224/5450576/Immobilization-of-Aspergillus-oryzae-IPT-301-Cells",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2916|Microbial Metabolites in Food Biotechnology"
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  "openalex": "W7221834620",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c07568",
  "pmcid": "PMC13718208",
  "pmid": "42960106",
  "published": "2026-10-14",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/a9zafpw0k701e.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c07568",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/42/65224/5450576/Immobilization-of-Aspergillus-oryzae-IPT-301-Cells"
    },
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  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Immobilization of Aspergillus oryzae IPT-301 Cells with High Transfructosylation Activity on Polyurethane Sponge for Fructooligosaccharide Production in Batch Mode",
  "title_text": "Immobilization of Aspergillus oryzae IPT-301 Cells with High Transfructosylation Activity on Polyurethane Sponge for Fructooligosaccharide Production in Batch Mode",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:18Z",
  "url": "https://research.draconitic.com/paper/a9zafpw0k701e",
  "venue": "ACS Omega",
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}