{
  "abstract": "Endophytic fungi represent potential sources of antifungal agents, but the phenotypic and chemical basis of their interactions with competing fungi remains incompletely understood. Here, physiological characterization, time-resolved confrontation assays, cell-free culture-filtrate testing, untargeted chemical profiling, and molecular docking were integrated to characterize the competitive phenotype of the endophytic fungus Diaporthe sp. PBS30-7, isolated from the medicinal plant Pereskia bleo (Kunth) DC., and to prioritize candidate metabolites for subsequent investigation. Diaporthe sp. PBS30-7 exhibited condition-dependent growth and a temporally asymmetric competitive phenotype against two Penicillium species, characterized by sustained colony expansion, progressive restriction of target-fungal growth, and pronounced hyphal abnormalities. Cell-free culture filtrates retained antifungal activity against both target fungi, supporting a contribution of extracellular chemical factors to the overall antagonistic phenotype. Feature-based molecular networking of a culture-associated agar–mycelium extract resolved a chemically diverse repertoire that included putatively annotated beauvericin-type cyclodepsipeptides, diketopiperazines, and unresolved molecular families. Molecular docking further prioritized this candidate chemical space, with beauvericin derivatives displaying particularly favorable target-dependent docking profiles toward CYP51, Fks1, and the ABC transporter, whereas cyclo(L-Tyr-L-Tyr) emerged as a notable diketopiperazine candidate. Together, these findings prioritize candidate metabolites potentially associated with the competitive phenotype of Diaporthe sp. PBS30-7 and provide a foundation for future mechanistic studies of endophytic fungal antagonism.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Universiti Malaya",
    "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universiti Malaya"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ling Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Chuancheng Wei"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/acb1xyb6hkabs.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1943254",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717619/?tool=EBI",
  "id": "acb1xyb6hkabs",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1943254/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2736|Microbial Natural Products and Biosynthesis"
    ]
  ],
  "openalex": "W7222033202",
  "pdf_url": "https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2026.1943254/pdf",
  "pmcid": "PMC13717619",
  "pmid": "42959819",
  "published": "2026-10-15",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/acb1xyb6hkabs.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1943254",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1943254/full"
    },
    {
      "first_seen": "2026-10-31T00:20:37Z",
      "native_id": "PMC:PMC13717619",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13717619"
    },
    {
      "first_seen": "2026-11-01T00:20:52Z",
      "native_id": "MED:42959819",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959819"
    },
    {
      "first_seen": "2026-11-02T12:21:54Z",
      "native_id": "PMC13717619",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fmicb.2026.1943254"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Microbial Natural Products and Biosynthesis",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Competitive phenotype and metabolite repertoire of the endophytic Diaporthe sp. PBS30-7",
  "title_text": "Competitive phenotype and metabolite repertoire of the endophytic Diaporthe sp. PBS30-7",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:54Z",
  "url": "https://research.draconitic.com/paper/acb1xyb6hkabs",
  "venue": "Frontiers in Microbiology",
  "versions": []
}