{
  "abstract": "Visualizing and troubleshooting long-read sequencing data is a common task when analyzing novel protocols, but identifying several primer sequences from reads exceeding 2 kb with fuzzy matching is challenging for the human eye. Here, we present seqsizzle, a cross-platform terminal user interface (TUI) based tool for convenient visualization of long-read sequencing data directly from the command-line, applicable to all sequencing technologies that produce FASTQ or FASTA output. seqsizzle allows primer sequences to be matched with user-specified fuzzy matching thresholds and highlighted with configurable colors. Additional styling can be applied to distinguish mismatches and quality scores, allowing convenient quality control of long-read sequencing data. To further aid troubleshooting, seqsizzle includes a built-in $k$-mer enrichment analysis, enabling the detection of unknown primer sequences or unexpected artifacts, which can then be reviewed via manual inspection. seqsizzle is implemented in Rust and is available open-source from https://github.com/ChangqingW/SeqSizzle.",
  "abstract_shown": true,
  "affiliations": [
    "Walter and Eliza Hall Institute of Medical Research"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "C. Wang"
    },
    {
      "name": "M. E. Ritchie"
    },
    {
      "name": "N. M. Davidson"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/acbsrej5958mk.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.07.17.738618",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.07.17.738618v2.full",
  "id": "acbsrej5958mk",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.07.17.738618v2",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioinformatics"
    ],
    [
      "openalex",
      "1312|Genomics and Phylogenetic Studies"
    ]
  ],
  "openalex": "W7170033057",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.07.17.738618v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-17",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/acbsrej5958mk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:30Z",
      "native_id": "10.64898/2026.07.17.738618",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.07.17.738618v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioinformatics",
      "subfield": "bioinformatics"
    }
  ],
  "title": "seqsizzle: decoding complex barcode and adapter architectures in long-read sequencing data",
  "title_text": "seqsizzle: decoding complex barcode and adapter architectures in long-read sequencing data",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:30Z",
  "url": "https://research.draconitic.com/paper/acbsrej5958mk",
  "venue": "bioRxiv",
  "versions": []
}