{
  "abstract": "Laguna Negra virus and Black Creek Canal virus are hantaviruses associated with hantavirus cardiopulmonary syndrome (HCPS), but their cellular entry receptors have not been defined. We used replication-competent recombinant vesicular stomatitis viruses (rVSVs) bearing hantavirus Gn/Gc glycoprotein complexes to compare entry by these viruses, the Old World rodent-borne Dabieshan virus and Tigray virus, and the shrew-borne Cao Bang virus and Amga virus. All six Gn/Gc complexes supported infection of primary human pulmonary microvascular endothelial cells, with virus-specific efficiencies and kinetics. Genetic ablation of protocadherin-1 (PCDH1) reduced infection mediated by Laguna Negra virus and Black Creek Canal virus Gn/Gc in human osteosarcoma and endothelial cells, whereas PCDH1 re-expression restored susceptibility. Both viruses were captured by a soluble PCDH1 protein comprising the first two extracellular cadherin domains. Infection was also inhibited by a soluble decoy containing the first cadherin domain, and by an antibody specific to that domain, indicating that the first PCDH1 cadherin domain mediates viral engagement. Black Creek Canal Gn/Gc-bearing rVSVs incorporated less Gn/Gc and showed lower apparent capture by PCDH1 than Laguna Negra virus and Andes virus Gn/Gc-bearing particles, even after normalization for glycoprotein abundance. In contrast, PCDH1 depletion did not detectably alter infection mediated by the other four hantavirus Gn/Gc complexes. Thus, Laguna Negra virus and Black Creek Canal virus use PCDH1 for efficient entry but differ in apparent receptor engagement and responses to PCDH1-directed inhibitors, whereas all six viruses are potently neutralized by the broadly reactive Gn/Gc-directed antibody ADI-42898.",
  "abstract_shown": true,
  "affiliations": [
    "Louisiana State University Health Sciences Center Shreveport School of Medicine"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "N. Guerra-Pilaquinga"
    },
    {
      "name": "C. Word"
    },
    {
      "name": "U. P. Lambe"
    },
    {
      "name": "L. Khera"
    },
    {
      "name": "R. Arinola"
    },
    {
      "name": "R. Kaur"
    },
    {
      "name": "M. E. Dieterle"
    },
    {
      "affs": [
        0
      ],
      "name": "R. K. Jangra"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/achyjep8yqa13.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.05.756634",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756634v1.full",
  "id": "achyjep8yqa13",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756634v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2725|Viral Infections and Vectors"
    ]
  ],
  "openalex": "W7220364416",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756634v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/achyjep8yqa13.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:07Z",
      "native_id": "10.64898/2026.10.05.756634",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756634v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Differential PCDH1 usage by Laguna Negra and Black Creek Canal hantaviruses",
  "title_text": "Differential PCDH1 usage by Laguna Negra and Black Creek Canal hantaviruses",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:07Z",
  "url": "https://research.draconitic.com/paper/achyjep8yqa13",
  "venue": "bioRxiv",
  "versions": []
}