{
  "abstract": "Respiratory burst oxidase homolog (RBOH)–based ROS production was widely verified playing vital roles in plant stress responses; however, its inhibitory mechanism remains largely unknown. Here, we demonstrate that a calmodulin-like (CML) protein OsCML27 functions as a flexible brake of OsRbohB-mediated ROS production to regulate rice drought tolerance via an OsMRLK63-OsCML27-OsRbohB module. OsCML27 negatively regulates drought tolerance of rice where the overexpression lines showed a faster water loss rate and a lower survival rate under drought, while the oscml27 mutants exhibited a slower dehydration rate and a slightly lower survival rate as compared to wild type. OsCML27 can interact with and be phosphorylated by a receptor-like kinase OsMRLK63 primarily at Ser 11 , and the phosphorylation level could be increased by drought. The phosphorylation by OsMRLK63 enhances OsCML27 stability, and OsCML27 inhibits ROS production by interacting with OsRbohB in the Ca 2+ -dependent manner. All these results reveal that OsCML27 integrates phosphorylation and Ca 2+ signals to regulate drought tolerance of rice by fine-tuning OsRbohB-mediated ROS signaling.",
  "abstract_shown": true,
  "affiliations": [
    "Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Qinhuangdao 066600, Hebei, China",
    "State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China",
    "College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China",
    "Institute of Quality Standards and Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China",
    "Hanbin District Agricultural Technology Extension Station, Ankang 725000, Shaanxi, China",
    "Qinyeqing Agricultural Development Co. Ltd, Ankang 725000, Shaanxi, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Peng‐Tao Shi",
      "orcid": "0009-0009-6128-8240"
    },
    {
      "affs": [
        2
      ],
      "name": "Ran Zhang",
      "orcid": "0009-0004-5970-4640"
    },
    {
      "affs": [
        1
      ],
      "name": "Xuan-Jie Wu",
      "orcid": "0009-0006-2922-9978"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhe Zhang",
      "orcid": "0000-0003-0632-7672"
    },
    {
      "affs": [
        3
      ],
      "name": "Binbin Li",
      "orcid": "0000-0002-8572-8003"
    },
    {
      "affs": [
        4
      ],
      "name": "Si-Lin Wang"
    },
    {
      "affs": [
        5
      ],
      "name": "Hao Chen"
    },
    {
      "affs": [
        4
      ],
      "name": "Xiu-Ni Fan",
      "orcid": "0009-0001-5958-8473"
    },
    {
      "affs": [
        1
      ],
      "name": "Wen-Ting Liu",
      "orcid": "0000-0001-7157-3277"
    },
    {
      "affs": [
        1
      ],
      "name": "Jingjing Wen",
      "orcid": "0009-0001-4673-5286"
    },
    {
      "affs": [
        1
      ],
      "name": "Kun‐Ming Chen",
      "orcid": "0000-0002-0016-9995"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/acwhmmy447v7t.bib",
  "cited_by": 5,
  "doi": "10.1126/sciadv.aee6303",
  "fulltext_url": null,
  "id": "acwhmmy447v7t",
  "issns": [
    "2375-2548"
  ],
  "landing_url": "https://doi.org/10.1126/sciadv.aee6303",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
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  "openalex": "W7166163011",
  "pdf_url": "https://www.science.org/doi/pdf/10.1126/sciadv.aee6303?download=true",
  "pmcid": "PMC13308589",
  "pmid": "42361178",
  "published": "2026-06-26",
  "publisher": "American Association for the Advancement of Science",
  "ris": "https://research.draconitic.com/paper/acwhmmy447v7t.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T13:20:45Z",
      "native_id": "W7166163011",
      "source": "openalex",
      "url": "https://doi.org/10.1126/sciadv.aee6303"
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  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling",
  "title_text": "A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:09Z",
  "url": "https://research.draconitic.com/paper/acwhmmy447v7t",
  "venue": "Science Advances",
  "versions": []
}