{
  "abstract": "Inflammatory bowel disease (IBD) is associated with intestinal inflammation and oxidative stress. Protein-rich crayfish shell (CS) by-products may provide a sustainable source of bioactive hydrolysates. This study screened five protease-derived hydrolysates and evaluated the alkaline protease-derived hydrolysate (ACSP) in lipopolysaccharide-stimulated IEC-6 cells and mice with DSS-induced colitis. ACSP scavenged DPPH and ABTS•⁺ radicals, with IC₅₀ values of 0.888 ± 0.004 and 0.853 ± 0.003 mg/mL. It also enhanced superoxide dismutase and catalase activities, and inhibited NO release and albumin denaturation in vitro. In IEC-6 cells, ACSP increased viability, reduced iNOS, IL-1β, IL-6, and TNF-α expression, and increased IL-10 expression. In mice, ACSP attenuated weight loss, disease activity, colon shortening, histopathological injury, and goblet cell depletion. The high-dose group had disease activity index and histological scores of 3.60 ± 0.55 and 4.80 ± 0.40 compared with 9.40 ± 1.14 and 10.40 ± 0.80 in the model group. ACSP reduced pro-inflammatory cytokines, myeloperoxidase activity, and NO levels while restoring antioxidant enzyme activities and tight junction protein expression. Peptidomic analysis identified 516 peptide sequences, including peptides containing hydrophobic, acidic, and C-terminal basic residues. These findings support further investigation of ACSP for intestinal health applications and the value-added utilization of CS by-products.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life and Health Sciences, Hubei University of Technology, No. 28 Nanli Road, Wuhan 430068, China.",
    "College of Food Science and Engineering, Yangzhou University, Yangzhou 210095, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lei Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongmin Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jinyang Peng"
    },
    {
      "affs": [
        0
      ],
      "name": "Shaohao Lai"
    },
    {
      "affs": [
        0
      ],
      "name": "Zexin Zhao",
      "orcid": "0000-0001-8975-7664"
    },
    {
      "affs": [
        1
      ],
      "name": "Yongqi Yin"
    },
    {
      "affs": [
        0
      ],
      "name": "Jun Cai"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/acxz91m192wy8.bib",
  "cited_by": 0,
  "doi": "10.3390/foods15193389",
  "fulltext_url": "https://europepmc.org/articles/PMC13664394",
  "id": "acxz91m192wy8",
  "issns": [
    "2304-8158"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883496",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Protein Hydrolysis and Bioactive Peptides"
    ]
  ],
  "openalex": "W7214092224",
  "pdf_url": "https://europepmc.org/articles/PMC13664394?pdf=render",
  "pmcid": "PMC13664394",
  "pmid": "42883496",
  "published": "2026-09-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/acxz91m192wy8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883496",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883496"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Protein Hydrolysis and Bioactive Peptides",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Anti-Inflammatory Protein Hydrolysate Screened from Crayfish Shells Attenuates Colitis by Oxidative Stress Suppression, Inflammation Reduction and Barrier Reconstruction.",
  "title_text": "Anti-Inflammatory Protein Hydrolysate Screened from Crayfish Shells Attenuates Colitis by Oxidative Stress Suppression, Inflammation Reduction and Barrier Reconstruction.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/acxz91m192wy8",
  "venue": "Foods (Basel, Switzerland)",
  "versions": []
}