{
  "abstract": "Background: Point-of-care manufacturing of CAR-T cells could reduce cost and improve patient access, but few closed, automated systems support both flexible process development and GMP production. Methods and results: We describe LimCORE, a single-use consumable for cell incubation and processing, implemented in LimGROW (manual system) and LimONE (automated system). In LimGROW, T cells expanded 246 +/-18-fold over 10 days at optimized seeding density, versus 89 +/-9-fold in a G-Rex control, with similar viability (>90%) and phenotype. Process parameters directly transferred to LimONE, where automated, closed buoyancy-based CD3+ selection achieved 90.3 +/-3.5% purity and 59.2 +/-17.0% recovery in under two hours. Using this workflow, we manufactured CD19 CAR-T cells in an automated 7-day LimONE process requiring 155 minutes of operator intervention. LimONE manufactured CAR-T cells reached 42.6 +/-2.4-fold expansion (532 +/-29 M cells at harvest), with 43.6 +/-6.0% transduction efficiency and viability similar to G-Rex controls. CD4+/CD8+ ratios, CD8+ differentiation subsets, and cytotoxicity were comparable between platforms, while LimONE-manufactured cells showed increased metabolic fitness. Across CAR-T manufacturing runs on the LimONE, no hardware or software failures occurred, and automated liquid transfers and volume concentration stayed within +/-5% accuracy. Conclusions: These data show that LimCORE supports T cell expansion, selection, and complete CD19 CAR-T manufacturing with performance similar to existing platforms while reducing operator time, across R&D and GMP scale. Key words: Automation; CAR-T; Point of care; T cell manufacturing",
  "abstract_shown": true,
  "affiliations": [
    "Limula"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "C. Boudousquie"
    },
    {
      "name": "D. Migliorini"
    },
    {
      "name": "J. Esensten"
    },
    {
      "name": "M. Irving"
    },
    {
      "name": "J. Maillard"
    },
    {
      "name": "R. Vuillefroy de Silly"
    },
    {
      "name": "Y. Pierson"
    },
    {
      "name": "V. Widmer"
    },
    {
      "name": "M. Pedard"
    },
    {
      "name": "M. Morbidelli"
    },
    {
      "name": "N. Vannini"
    },
    {
      "name": "E. Nallet"
    },
    {
      "name": "L. Bellosta"
    },
    {
      "name": "L. Dutoit"
    },
    {
      "name": "F. Jammes"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/adxz2bx6m1p9v.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.08.748871",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.08.748871v1.full",
  "id": "adxz2bx6m1p9v",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.08.748871v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "2730|CAR-T cell therapy research"
    ]
  ],
  "openalex": "W7213133272",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.08.748871v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/adxz2bx6m1p9v.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:20Z",
      "native_id": "10.64898/2026.09.08.748871",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.08.748871v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Closed, Automated CAR-T Cell Manufacturing: from Research to Point of Care production.",
  "title_text": "Closed, Automated CAR-T Cell Manufacturing: from Research to Point of Care production.",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:00Z",
  "url": "https://research.draconitic.com/paper/adxz2bx6m1p9v",
  "venue": "bioRxiv",
  "versions": []
}