{
  "abstract": "Intracellular immune receptors protect plants from microbial invasion by detecting and responding to pathogen-derived effector molecules, often triggering cell death responses. However, pathogen effectors can evolve to avoid immune recognition, resulting in devastating diseases that threaten global agriculture. Here, we show that an integrated Exo70 domain from the barley intracellular nucleotide-binding leucine-rich repeat (NLR) RGH2 can interact with both the rice blast pathogen effector AVR-Pii and a closely related wheat blast causing variant. We used structure-led engineering to develop RGH2+ that shows increased binding affinity toward AVR-Pii variants and increased cell death responses on heterologous expression in Nicotiana benthamiana. Infection assays in transgenic barley lines carrying RGH2+ with the paired NLR RGH3 indicate a reduced susceptibility to blast strains expressing AVR-Pii variants. These results demonstrate the potential of engineering NLR receptors as an effective strategy for improving resistance toward one of the most destructive diseases affecting cereal production.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park , Norwich NR4 7UH ,",
    "John Innes Centre",
    "2Blades , Evanston, IL 60201 ,",
    "The Sainsbury Laboratory, University of East Anglia, Norwich Research Park , Norwich NR4 7UH ,",
    "The Sainsbury Laboratory",
    "Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park , Norwich NR4 7UH ,",
    "USDA-ARS Cereal Disease Laboratory , 1551 Lindig Street, Saint Paul, MN 55108 ,",
    "University of Minnesota",
    "Center for Plant Molecular Biology (ZMBP), Eberhard Karls Universität Tübingen , Tübingen 72076 ,",
    "Gregor Mendel Institute of Molecular Plant Biology"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Indira Saado",
      "orcid": "0000-0002-3462-0983"
    },
    {
      "affs": [
        2,
        3,
        4
      ],
      "name": "Helen J. Brabham",
      "orcid": "0000-0001-7132-5011"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "J. R. Bennett",
      "orcid": "0000-0001-8576-7195"
    },
    {
      "affs": [
        5,
        1
      ],
      "name": "Anson Ho Ching Lam",
      "orcid": "0000-0001-7805-2132"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Inmaculada Hernández‐Pinzón",
      "orcid": "0000-0003-3711-2893"
    },
    {
      "affs": [
        3,
        6,
        7
      ],
      "name": "Matthew Moscou",
      "orcid": "0000-0003-2098-6818"
    },
    {
      "affs": [
        8,
        0,
        3,
        9
      ],
      "name": "Juan Carlos De la Concepción",
      "orcid": "0000-0002-7642-8375"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Mark James Banfield",
      "orcid": "0000-0001-8921-3835"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/afvwa21ygz2wg.bib",
  "cited_by": 7,
  "doi": "10.1093/plcell/koag168",
  "fulltext_url": null,
  "id": "afvwa21ygz2wg",
  "issns": [
    "1040-4651",
    "1532-298X"
  ],
  "landing_url": "https://doi.org/10.1093/plcell/koag168",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant-Microbe Interactions and Immunity"
    ]
  ],
  "openalex": "W4405756817",
  "pdf_url": null,
  "pmcid": "PMC13291817",
  "pmid": "42263189",
  "published": "2026-06-01",
  "publisher": "Oxford University Press",
  "ris": "https://research.draconitic.com/paper/afvwa21ygz2wg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:50Z",
      "native_id": "W4405756817",
      "source": "openalex",
      "url": "https://doi.org/10.1093/plcell/koag168"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant-Microbe Interactions and Immunity",
      "subfield": "plant-biology"
    }
  ],
  "title": "Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance",
  "title_text": "Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:08Z",
  "url": "https://research.draconitic.com/paper/afvwa21ygz2wg",
  "venue": "The Plant Cell",
  "versions": []
}