{
  "abstract": "Vaccination induces durable adaptive immunity, yet how transcriptional and chromatin programs are dynamically established and reorganized across successive vaccine doses remains poorly understood. Here, we performed paired single-nucleus RNA sequencing and chromatin accessibility profiling of peripheral blood mononuclear cells collected from four individuals at ten time points spanning primary and booster immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. More differentially expressed genes and accessible regions were detected after primary than booster vaccination. Notably, independent public Hi-C data provided structural context for candidate DAR-gene associations. In B cells, AP-1 motif accessibility decreased dynamically after primary vaccination, followed by increased plasma cell differentiation and secretory programs. In T cells, signature scores decreased on day 1 after primary vaccination and were accompanied by remodeling of transcription factor-associated chromatin accessibility. These intracellular changes were accompanied by temporal reorganization of T and B cell communication and the emergence of distinct multicellular modules associated with primary and booster responses. Together, our findings describe temporal changes in gene expression and chromatin accessibility across circulating T and B cells following primary and booster vaccination in this cohort.",
  "abstract_shown": true,
  "affiliations": [
    "Bio-X Center for Interdisciplinary Innovation, Yunnan University, Kunming 650091, China",
    "Yunnan Vaccine Laboratory, Kunming 650091, China",
    "Southwest United Graduate School, Kunming 650092, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Rong Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Huajie Hu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuan Li"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Zijie Scott Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Rui Cheng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ag27sphxt564d.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111608",
  "fulltext_url": null,
  "id": "ag27sphxt564d",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1608",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-04",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/ag27sphxt564d.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:20:58Z",
      "native_id": "10.3390/biom16111608",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1608"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-273X",
      "subfield": "general"
    }
  ],
  "title": "Single-Cell Multi-Omics Reveals Dynamic Chromatin Remodeling of Adaptive Immunity During Prime-Boost Vaccination",
  "title_text": "Single-Cell Multi-Omics Reveals Dynamic Chromatin Remodeling of Adaptive Immunity During Prime-Boost Vaccination",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T00:20:58Z",
  "url": "https://research.draconitic.com/paper/ag27sphxt564d",
  "venue": "Biomolecules",
  "versions": []
}