{
  "abstract": "Redox-related molecules, such as quinones and reactive oxygen species (ROS), are important signaling molecules for all living organisms. A plant-specific leucine rich-repeat receptor-like kinase (LRR-RLK) CANNOT RESPOND TO DMBQ 1 (CARD1), also known as HYDROGEN-PEROXIDE-INDUCED Ca2+ INCREASES (HPCA1), perceives both quinones and ROS, but knowledge on key structural features is unknown. Here, we determine the structure of the CARD1 ectodomain and uncover its unique features. Structural studies, coupled with genetics and biochemical analysis, demonstrated that previously identified unique cysteine residues are not essential for signal perception in CARD1. Interestingly, CARD1 harbors a copper ion on the surface of the ectodomain via histidine-coordination that is crucial for hydrogen peroxide signaling. Our work reports the first copper-dependent redox receptor and highlights a unique perspective between a receptor and non-peptide stimuli during their perception.",
  "abstract_shown": true,
  "affiliations": [
    "RIKEN Center for Sustainable Resource Science, Yokohama, Japan",
    "Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science",
    "Yokohama, Kanagawa, 230-0045, Japan",
    "Graduate School of Pharmaceutical Sciences, The University of Osaka, Suita, Japan",
    "Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), The University of Osaka, Suita, Japan",
    "Graduate School of Pharmaceutical Sciences, Osaka University",
    "Suita, Osaka, 565-0871, Japan",
    "Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan",
    "Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University",
    "Nagoya, Aichi, 464-8601, Japan",
    "THERS Technical Center, Nagoya University, Nagoya, Japan",
    "Nagoya, Aichi, 6-8603, Japan",
    "THERS Technical Center, Nagoya University",
    "Center for Infectious Disease Education and Research (CiDER), The University of Osaka, Suita, Japan",
    "Department of Biological Science, Graduate School of Science, The University of Tokyo, Bunkyo, Japan",
    "Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan. laohavisit.anuphon.f2@f.mail.nagoya-u.ac.jp"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Nobuaki Ishihama",
      "orcid": "0000-0001-5331-1599"
    },
    {
      "affs": [
        3,
        4,
        5,
        6
      ],
      "name": "Yohta Fukuda",
      "orcid": "0000-0002-7386-8201"
    },
    {
      "affs": [
        7,
        8,
        9
      ],
      "name": "Yumiko Shirano"
    },
    {
      "affs": [
        7,
        1,
        2
      ],
      "name": "Kazuhiro J. Fujimoto",
      "orcid": "0000-0003-0286-3646"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Kaori Takizawa"
    },
    {
      "affs": [
        0,
        8,
        9
      ],
      "name": "Ryoko Hiroyama"
    },
    {
      "affs": [
        10,
        11,
        12
      ],
      "name": "Hiroki Ito",
      "orcid": "0000-0002-4433-370X"
    },
    {
      "affs": [
        10,
        11,
        12
      ],
      "name": "Mayumi Nishimura"
    },
    {
      "affs": [
        7,
        8,
        9
      ],
      "name": "Takeshi Yanai",
      "orcid": "0000-0003-3933-8546"
    },
    {
      "affs": [
        13,
        3,
        4,
        5,
        6
      ],
      "name": "Tsuyoshi Inoue A",
      "orcid": "0000-0001-5337-5066"
    },
    {
      "affs": [
        14,
        0,
        1,
        2
      ],
      "name": "Ken Shirasu",
      "orcid": "0000-0002-0349-3870"
    },
    {
      "affs": [
        15,
        8,
        9
      ],
      "name": "Anuphon Laohavisit",
      "orcid": "0000-0002-7707-8928"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/agw1zr7tmwjk5.bib",
  "cited_by": 6,
  "doi": "10.1038/s41467-026-72573-8",
  "fulltext_url": null,
  "id": "agw1zr7tmwjk5",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://doi.org/10.1038/s41467-026-72573-8",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant-Microbe Interactions and Immunity"
    ]
  ],
  "openalex": "W4412715415",
  "pdf_url": "https://www.nature.com/articles/s41467-026-72573-8.pdf",
  "pmcid": "PMC13184263",
  "pmid": "42151134",
  "published": "2026-05-18",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/agw1zr7tmwjk5.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T13:21:09Z",
      "native_id": "W4412715415",
      "source": "openalex",
      "url": "https://doi.org/10.1038/s41467-026-72573-8"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant-Microbe Interactions and Immunity",
      "subfield": "plant-biology"
    }
  ],
  "title": "A copper-dependent redox-based hydrogen peroxide perception in plants",
  "title_text": "A copper-dependent redox-based hydrogen peroxide perception in plants",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:09Z",
  "url": "https://research.draconitic.com/paper/agw1zr7tmwjk5",
  "venue": "Nature Communications",
  "versions": []
}