{
  "abstract": "Hepatocellular carcinoma (HCC) arises in heterogeneous etiological contexts, but direct cross-omics comparisons between HCC subgroups defined by different etiologies remain limited. In this exploratory study, we combined fecal 16S rRNA gene sequencing and untargeted serum metabolomics with exploratory cross-omics analyses to compare gut microbial composition, serum metabolic profiles, and microbiome–metabolite association patterns between hepatitis B virus-related HCC (HBV-HCC) and alcohol-related HCC (ALD-HCC). Forty patients who underwent primary tumor resection were included, comprising 26 with HBV-HCC and 14 with ALD-HCC. Alpha diversity indices did not differ significantly between groups, whereas within-group Bray–Curtis dissimilarity was greater in ALD-HCC and genus-level beta-diversity ordination showed only modest separation between the two groups. LEfSe-based screening suggested the enrichment of candidate taxa, including Faecalibacterium , Roseburia , Lachnospira , Turicibacter , and Butyribacter in HBV-HCC, whereas Allisonella and Coprobacillus were enriched in ALD-HCC. Untargeted metabolomics showed group separation in supervised models and identified exploratory candidate metabolic features mainly involving glycerophospholipid metabolism, primary bile acid biosynthesis, aminoacyl-tRNA biosynthesis, and central carbon metabolism in cancer; however, these metabolite-level findings did not remain significant after multiple-testing correction. Procrustes and O2PLS analyses indicated limited global concordance between the microbiome and metabolome, although exploratory associations were observed between several phosphatidylcholine-related features and microbial taxa. Overall, HBV-HCC and ALD-HCC may be associated with different gut microbial and serum metabolic patterns, and these findings should be considered exploratory and hypothesis-generating.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University , , ,",
    "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University , ,",
    "Guangxi Medical University , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yongguang Wei"
    },
    {
      "affs": [
        1
      ],
      "name": "Kejian Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Qinchen Lu"
    },
    {
      "affs": [
        1
      ],
      "name": "Xin Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Huasheng Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Meiliang Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijun Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Liming Shang"
    },
    {
      "affs": [
        1
      ],
      "name": "Tao Peng"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaoyun Zeng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ajb3p8w0bq6zd.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1927595",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671147/?tool=EBI",
  "id": "ajb3p8w0bq6zd",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13671147",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Gut microbiota and health"
    ]
  ],
  "openalex": "W7214901617",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13671147/pdf/?tool=EBI",
  "pmcid": "PMC13671147",
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/ajb3p8w0bq6zd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:18Z",
      "native_id": "PMC:PMC13671147",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13671147"
    },
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1927595",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1927595/full"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Gut microbiota and health",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Etiology-associated differences in the gut microbiome and metabolome in hepatocellular carcinoma: an exploratory study of alcohol-related and HBV-related cases",
  "title_text": "Etiology-associated differences in the gut microbiome and metabolome in hepatocellular carcinoma: an exploratory study of alcohol-related and HBV-related cases",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:12Z",
  "url": "https://research.draconitic.com/paper/ajb3p8w0bq6zd",
  "venue": "Frontiers in microbiology",
  "versions": []
}