{
  "abstract": "Background: Dengue virus serotype 1 (DENV-1) is prevalent across the Americas, yet its genomic diversity in Mexico is poorly characterized. Methods: We analyzed 119 public Mexican DENV-1 genomes (polyprotein CDS, 10,179 nt) alongside 111 from 19 other American countries and territories. The Mexican panel was temporally and geographically uneven (91% from 2006 to 2009), representing available sequences, not nationwide diversity. We assessed diversity, phylogeny, dating, selection, recombination, and compatibility with three primer sets. Results: The Mexican genotype V sequences formed 115 haplotypes (Hd = 0.9994; π = 0.00831), resolving into clades A (n = 6) and B (n = 112) plus one independent introduction in Jalisco. These sequences were not monophyletic: both clades grouped with Nicaraguan and United States viruses. Their common ancestor dated to 1977.5 and clade divergence to 1984.2. Selection appeared to be predominantly purifying (ω = 0.037); no site showed concordant evidence of diversifying selection, and no recombination was detected. In silico, 84% of Mexican sequences were compatible with all three CDC real-time oligonucleotides; none matched every oligonucleotide in either conventional set. Conclusions: This historical panel shows substantial haplotype diversity and multiple introductions. The compatibility results are computational and were not validated experimentally. We recommend periodic in silico primer revalidation.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Conservation Medicine, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa 88700, Tamaulipas, Mexico.",
    "UAM Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa 88740, Tamaulipas, Mexico."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Francisco Joel Cabrera-Santos",
      "orcid": "0009-0001-6305-9216"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Fátima Yedith Camacho-Sánchez",
      "orcid": "0000-0001-5557-2565"
    },
    {
      "affs": [
        0
      ],
      "name": "Sorayda Leonela González-Trujillo",
      "orcid": "0009-0001-5234-8944"
    },
    {
      "affs": [
        0
      ],
      "name": "Miguel Angel Reyes-López",
      "orcid": "0000-0001-9939-3032"
    },
    {
      "affs": [
        0
      ],
      "name": "Erick de Jesús De Luna-Santillana",
      "orcid": "0000-0003-1257-5832"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/amy2qez8s4kvq.bib",
  "cited_by": 0,
  "doi": "10.3390/tropicalmed11100287",
  "fulltext_url": "https://europepmc.org/articles/PMC13709455",
  "id": "amy2qez8s4kvq",
  "issns": [
    "2414-6366"
  ],
  "landing_url": "https://europepmc.org/article/MED/42931472",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2739|Mosquito-borne diseases and control"
    ]
  ],
  "openalex": "W7207858340",
  "pdf_url": "https://europepmc.org/articles/PMC13709455?pdf=render",
  "pmcid": "PMC13709455",
  "pmid": "42931472",
  "published": "2026-10-12",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/amy2qez8s4kvq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:01Z",
      "native_id": "MED:42931472",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42931472"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Mosquito-borne diseases and control",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Genomic Diversity, Phylogenetic Structure, and In Silico Diagnostic Primer Compatibility of Available DENV-1 Genotype V from Mexico.",
  "title_text": "Genomic Diversity, Phylogenetic Structure, and In Silico Diagnostic Primer Compatibility of Available DENV-1 Genotype V from Mexico.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:01Z",
  "url": "https://research.draconitic.com/paper/amy2qez8s4kvq",
  "venue": "Tropical medicine and infectious disease",
  "versions": []
}