{
  "abstract": "Human Merkel cell polyomavirus (MCV) large T (LT) protein functions as a replication helicase analogous to the cellular MCM complex but requires a second MCV protein, small T (sT), for efficient LT-initiated replication. Both LT and sT encode highly-conserved DnaJ domains that bind to the cellular refolding molecule, heat shock protein 70 (HSP70). Using single-molecule imaging and FRET, we find that sT recruits dimeric HSP70 to serve as a molecular bridge between sT and LT on DNA and that this complex facilitates remodeling LT into a functional double-hexameric helicase. Disruption of LT-HSP70-HSP70-sT interaction with HSP70 inhibitors or by mutations to the LT-sT DnaJ domains collapse the LT-HSP70-HSP70-sT complex, abolishing sT-enhanced LT dodecamerization and ATP-dependent DNA unwinding. Assembly of virus-free mammalian MCM3, MCM4 and MCM6 in nuclear extracts similarly recruits HSP70 to DNA suggesting that Hsp70 remodeling is used for efficient eukaryotic assembly of the multi-protein replication machinery.",
  "abstract_shown": true,
  "affiliations": [
    "University of Pittsburgh"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "L. Wan"
    },
    {
      "name": "X. Li"
    },
    {
      "name": "P. Vittot"
    },
    {
      "name": "J. Brodsky"
    },
    {
      "name": "P. Wipf"
    },
    {
      "name": "P. S. Moore"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Chang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/an47rcx56rf3x.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.11.05.764654",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.11.05.764654v1.full",
  "id": "an47rcx56rf3x",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.11.05.764654v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.11.05.764654v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/an47rcx56rf3x.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:11Z",
      "native_id": "10.64898/2026.11.05.764654",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.11.05.764654v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A Human Tumor Virus Recruits HSP70 Chaperone to Remodel Its Replisome on DNA",
  "title_text": "A Human Tumor Virus Recruits HSP70 Chaperone to Remodel Its Replisome on DNA",
  "type": "preprint",
  "updated_at": "2026-11-10T00:22:56Z",
  "url": "https://research.draconitic.com/paper/an47rcx56rf3x",
  "venue": "bioRxiv",
  "versions": []
}