{
  "abstract": "The Ralstonia solanacearum species complex (RSSC) causes bacterial wilt in a broad range of economically important hosts. The RSSC has been reclassified into three species, of which R. solanacearum sequevars 1 and 2 in phylotype IIB are designated as Select Agents in the U.S. Many rapid tests for “R. solanacearum” target the RSSC and thus cross-react with R. pseudosolanacearum, which is endemic to the U.S. To avoid false positive identifications and unnecessary triggering of Select Agent measures, four portable LAMP-based screening assay variations were developed and validated for specific detection of R. pseudosolanacearum: real-time LAMP assays for detection of R. pseudosolanacearum (1) and differentiation of R. pseudosolanacearum phylotypes I and III in the AmpliFire® Isothermal Fluorometer (2) or Genie® III (3), and a colorimetric LAMP for detection of R. pseudosolanacearum (4). All assays demonstrated high sensitivity (10–50 colony-forming units (CFU)/reaction) and specificity; 100% analytical specificity and selectivity based on 177 RSSC isolates (81 phylotype I, 70 phylotype II, 15 phylotype III and 11 phylotype IV) and 53 non-RSSC bacterial pathogens on relevant hosts and soil, and 100% diagnostic specificity and sensitivity based on testing inoculated plants and a Euphresco sample panel. The assays presented here represent useful diagnostic tools that can be deployed on-site to aid domestic survey and detection efforts.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27607, USA",
    "Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), Science and Technology (S&T), Plant Protection and Quarantine (PPQ), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Laurel, MD 20708, USA",
    "Floral and Nursery Plants Research Unit, United States National Arboretum, USDA Agricultural Research Service (ARS), Beltsville, MD 20705, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Brijesh B. Karakkat"
    },
    {
      "affs": [
        2
      ],
      "name": "Qi Huang"
    },
    {
      "affs": [
        1
      ],
      "name": "Schyler O. Nunziata"
    },
    {
      "affs": [
        1
      ],
      "name": "Jarred Yasuhara-Bell",
      "orcid": "0000-0001-5365-8170"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/anv4b4t2fv5k5.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27208976",
  "fulltext_url": "https://europepmc.org/articles/PMC13704481",
  "id": "anv4b4t2fv5k5",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/8976",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Pathogenic Bacteria Studies"
    ]
  ],
  "openalex": "W7221344657",
  "pdf_url": "https://europepmc.org/articles/PMC13704481?pdf=render",
  "pmcid": "PMC13704481",
  "pmid": "42941752",
  "published": "2026-10-10",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/anv4b4t2fv5k5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27208976",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/8976"
    },
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42941752",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42941752"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Pathogenic Bacteria Studies",
      "subfield": "plant-biology"
    }
  ],
  "title": "Genome-Informed Loop-Mediated Isothermal Amplification (LAMP) Assays for Detection and Differentiation of Ralstonia pseudosolanacearum Phylotypes I and III",
  "title_text": "Genome-Informed Loop-Mediated Isothermal Amplification (LAMP) Assays for Detection and Differentiation of Ralstonia pseudosolanacearum Phylotypes I and III",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/anv4b4t2fv5k5",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}