{
  "abstract": "Thienothiazolocarboxamides (TTCs) were discovered as a hit in a whole cell screen for new antituberculosis agents. These molecules were rapidly cidal under both replicating and non-replicating conditions and killed Mycobacterium tuberculosis (Mtb) in macrophages. The heterobicyclic core of this scaffold was essential for activity and resistance to these agents was mediated by loss of EthA, a flavin containing monooxygenase also responsible for the bioactivation of ethionamide. EthA-mediated activation of TTCs in whole cells was found to produce mycothiol adducts and RNAseq profiles revealed a mechanism of action identical to the fungal mycotoxins patulin and nidulalin A. EthA oxidizes the sulfur of the thiazole ring to create a relatively stable sulfenic acid that reacts with cellular thiols. This unique, replication-independent, mechanism suggests the possibility that these molecules may contribute significantly to shortening the duration of tuberculosis chemotherapy.",
  "abstract_shown": true,
  "affiliations": [
    "Tuberculosis Research Section, LCID, NIAID, NIH"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "name": "S. Oh"
    },
    {
      "name": "G. Prosser"
    },
    {
      "name": "H. I. M. Boshoff"
    },
    {
      "name": "S. R. Green"
    },
    {
      "name": "N. Quadir"
    },
    {
      "name": "J. S. O. Roma"
    },
    {
      "name": "A. Kumar"
    },
    {
      "name": "N. Mukundan"
    },
    {
      "name": "S. Hanson"
    },
    {
      "name": "J. P. Sarathy"
    },
    {
      "name": "K. I. Buchanan"
    },
    {
      "name": "P. C. Ray"
    },
    {
      "name": "P. G. Wyatt"
    },
    {
      "name": "T. R. Ioerger"
    },
    {
      "name": "A. L. Schwan"
    },
    {
      "name": "L. A. T. Cleghorn"
    },
    {
      "affs": [
        0
      ],
      "name": "C. E. Barry"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ap1d3qx4xgtdr.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.11.06.764870",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.11.06.764870v1.full",
  "id": "ap1d3qx4xgtdr",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.11.06.764870v1",
  "language": null,
  "licence": "CC0-1.0",
  "licence_label": "CC0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/publicdomain/zero/1.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.11.06.764870v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ap1d3qx4xgtdr.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T00:27:11Z",
      "native_id": "10.64898/2026.11.06.764870",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.11.06.764870v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Thienothiazolocarboxamides: rapidly cidal agents causing catastrophic thiol collapse in Mycobacterium tuberculosis.",
  "title_text": "Thienothiazolocarboxamides: rapidly cidal agents causing catastrophic thiol collapse in Mycobacterium tuberculosis.",
  "type": "preprint",
  "updated_at": "2026-11-10T00:27:11Z",
  "url": "https://research.draconitic.com/paper/ap1d3qx4xgtdr",
  "venue": "bioRxiv",
  "versions": []
}