{
  "abstract": "Here, we present a comprehensive methodology for real-time visualization of fluorescently tagged target proteins in living bacteria. We detail the use of micro-fabricated devices, such as agarose pads and microfluidic systems, to sustain cell viability and enable precise environmental control, ensuring accurate and reproducible imaging conditions. The chapter covers sample preparation, microscope setup, image acquisition, and the analysis of fluorescence dynamics at the single-cell level.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Perrine Revoil"
    },
    {
      "name": "Jérôme Rech"
    },
    {
      "name": "Jean-Yves Bouet"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/aq32jys81v5a0.bib",
  "cited_by": 0,
  "doi": null,
  "fulltext_url": null,
  "id": "aq32jys81v5a0",
  "issns": [],
  "landing_url": "https://hal.science/hal-05064515v2",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://hal.science/hal-05064515/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/aq32jys81v5a0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "hal-05064515",
      "source": "hal",
      "url": "https://hal.science/hal-05064515v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "Live-Imaging of Molecular Processes with Real-Time Fluorescence Microscopy",
  "title_text": "Live-Imaging of Molecular Processes with Real-Time Fluorescence Microscopy",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/aq32jys81v5a0",
  "venue": "HAL",
  "versions": []
}