{
  "abstract": "Rheumatoid arthritis (RA), a chronic autoimmune condition, causes irreversible bone destruction. A disintegrin and metalloprotease 10 (ADAM-10) has been implicated in inflammatory processes in RA. We investigated the potential role of ADAM-10 in osteoblast mineralization and RA-associated bone destruction. Serum ADAM-10 levels in RA patients were measured by ELISA, and ADAM-10 expression in osteoblasts was assessed by Western blotting. Collagen-induced arthritis (CIA) mice were treated intraperitoneally with the ADAM-10 inhibitor GI254023X (100 mg/kg) for three weeks. Arthritis and bone destruction were evaluated by macroscopic, immunohistochemical, histological, and micro-CT analyses. The effects of ADAM-10 inhibition on primary osteoblast mineralization were also assessed by Alizarin Red S staining. Serum ADAM-10 levels in patients with RA tended to be higher at Stage III of the Steinbrocker classification. In CIA mice, ADAM-10 inhibition attenuated weight loss, paw swelling, and arthritis scores and suppressed bone destruction. ADAM-10 inhibition also significantly reduced Rankl expression in knee joints. Furthermore, the inhibition reversed the suppression of primary osteoblast mineralization induced by TNF-α. Pharmacological inhibition of ADAM-10 attenuated experimental arthritis and bone destruction and improved osteoblast mineralization, suggesting that ADAM-10 may contribute to inflammatory bone destruction in RA.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pathogenesis and Translational Medicine, Showa Medical University Graduate School of Pharmacy, Tokyo 142-8555, Japan",
    "Division of Rheumatology, Department of Medicine, Showa Medical University, Tokyo 142-8555, Japan",
    "Division of Medical Pharmacology, Department of Pharmacology, Showa Medical University School of Medicine, Tokyo 142-8555, Japan",
    "Pharmacological Research Center, Showa Medical University, Tokyo 142-8555, Japan",
    "Department of Pharmacology, Graduate School of Dentistry, Showa Medical University, Tokyo 142-8555, Japan",
    "Department of Microbiology and Immunology, Showa Medical University Graduate School of Medicine, Tokyo 142-8555, Japan",
    "Division of Nephrology, Department of Medicine, Showa Medical University Graduate School of Medicine, Tokyo 142-8555, Japan"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Haruka Nakamura"
    },
    {
      "affs": [
        0
      ],
      "name": "Hiroyuki Kojima",
      "orcid": "0009-0000-3104-5154"
    },
    {
      "affs": [
        0
      ],
      "name": "Kohei Maeda",
      "orcid": "0000-0003-4195-6183"
    },
    {
      "affs": [
        0
      ],
      "name": "Kosuke Sakurai",
      "orcid": "0000-0001-5376-4707"
    },
    {
      "affs": [
        0
      ],
      "name": "Toshihiro Tanioka"
    },
    {
      "affs": [
        1
      ],
      "name": "Sho Ishii"
    },
    {
      "affs": [
        1
      ],
      "name": "Kuninobu Wakabayashi",
      "orcid": "0000-0002-9554-4742"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Masahiro Hosonuma"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Yuki Azetsu"
    },
    {
      "affs": [
        5,
        6
      ],
      "name": "Masayuki Iyoda",
      "orcid": "0000-0002-1153-3000"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Masamichi Takami"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Takeo Isozaki",
      "orcid": "0000-0002-8621-7643"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/aqmh05wstbxcz.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219842",
  "fulltext_url": null,
  "id": "aqmh05wstbxcz",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9842",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/aqmh05wstbxcz.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:20:58Z",
      "native_id": "10.3390/ijms27219842",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9842"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "ADAM-10 Is Involved in Osteoblast Mineralization and Rheumatoid Arthritis-Associated Bone Destruction",
  "title_text": "ADAM-10 Is Involved in Osteoblast Mineralization and Rheumatoid Arthritis-Associated Bone Destruction",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T00:21:14Z",
  "url": "https://research.draconitic.com/paper/aqmh05wstbxcz",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}