{
  "abstract": "Murine research on atherosclerosis has been conducted largely on the C57BL/6 (B6) strain background, and most commonly, the B6.129P2-Apoetm1Unc/J (B6-ApoEKO) strain. The use of the B6 background strain limits translatability for immunotherapy development due to inherent species differences between human and mouse immune systems. A proposed solution to this limitation is a humanized mouse model of atherosclerosis that would support the engraftment of human lymphoid and myeloid cell populations. The development of such a model has been hindered by the lack of a mouse strain that both develops atherosclerosis and supports human immune cell engraftment. In this paper, we report on a NOD.Cg-PrkdcscidIl2rgtm1Wjl/Sz (NSG) immunodeficient mouse strain that has a 383 bp deletion in the Apoe gene which prevents ApoE protein expression. When maintained on a high fat diet, NSG-ApoEKO mice develop hypercholesteremia and atherosclerosis at levels similar to B6-ApoEKO mice. While atherosclerosis in B6-ApoEKO mice has been widely studied in many laboratories under multiple conditions, our initial study of disease development in NSG-ApoEKO mice was limited and future comparative studies with B6-ApoEKO mice are necessary. NSG-ApoEKO mice concomitantly support human cell engraftment while developing atherosclerosis, thereby making them useful to study human-specific immune aspects.",
  "abstract_shown": true,
  "affiliations": [
    "The Jackson Laboratory, Bar Harbor, Maine, USA.",
    "Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.",
    "Program in Molecular Medicine, Diabetes Center of Excellence, UMass Chan Medical School, Worcester, Massachusetts, USA."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Hannah R Megathlin",
      "orcid": "0000-0002-8934-7654"
    },
    {
      "affs": [
        0
      ],
      "name": "Lisa Burzenski",
      "orcid": "0000-0002-7767-2345"
    },
    {
      "affs": [
        0
      ],
      "name": "David A Coleman",
      "orcid": "0009-0000-6459-8599"
    },
    {
      "affs": [
        0
      ],
      "name": "Blaze Albert",
      "orcid": "0009-0006-5113-5417"
    },
    {
      "affs": [
        0
      ],
      "name": "Michelle L Farley",
      "orcid": "0009-0001-1376-8495"
    },
    {
      "affs": [
        2
      ],
      "name": "Michael A Brehm",
      "orcid": "0000-0001-6813-3262"
    },
    {
      "affs": [
        2
      ],
      "name": "Dale L Greiner",
      "orcid": "0000-0002-5923-339X"
    },
    {
      "affs": [
        0
      ],
      "name": "Leonard D Shultz",
      "orcid": "0000-0002-4868-1031"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/arb5aarthjv3b.bib",
  "cited_by": 0,
  "doi": "10.1096/fj.202603399r",
  "fulltext_url": "https://europepmc.org/articles/PMC13686551",
  "id": "arb5aarthjv3b",
  "issns": [
    "0892-6638",
    "1530-6860"
  ],
  "landing_url": "https://europepmc.org/article/MED/42905940",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Atherosclerosis and Cardiovascular Diseases"
    ]
  ],
  "openalex": "W7223107524",
  "pdf_url": "https://europepmc.org/articles/PMC13686551?pdf=render",
  "pmcid": "PMC13686551",
  "pmid": "42905940",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/arb5aarthjv3b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42905940",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42905940"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Atherosclerosis and Cardiovascular Diseases",
      "subfield": "immunology"
    }
  ],
  "title": "NSG Mice Lacking Apoe: An Immunodeficient Mouse Model of Atherosclerosis.",
  "title_text": "NSG Mice Lacking Apoe: An Immunodeficient Mouse Model of Atherosclerosis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/arb5aarthjv3b",
  "venue": "FASEB journal : official publication of the Federation of American Societies for Experimental Biology",
  "versions": []
}