{
  "abstract": "Paclitaxel is a highly valuable diterpenoid anticancer drug originally extracted from the yew tree. Due to the low efficiency of plant extraction and the high cost of semi-synthesis, efforts have been made to produce paclitaxel through biosynthesis. However, there have been no reports to date on engineered strains capable of paclitaxel biosynthesis. Here, we engineer a paclitaxel-producing line using the moss ( Physcomitrium patens ) chassis cells by combining transient expression verification, cytoplasmic localization strategies, self-cleaving 2 A peptide-mediated multi-gene co-expression systems and a modular iterative transformation strategy for stable genetic inheritance. Even after continuous subculturing multiple times, the engineered paclitaxel-producing lines obtained through both the initial reconstructed pathway and the optimized pathway are able to stably produce paclitaxel. This study proposes that Physcomitrium patens can serve as a plant chassis for paclitaxel biosynthesis.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "Mengkai Zhao",
      "orcid": "0000-0002-7647-3520"
    },
    {
      "name": "Wenchang Lu"
    },
    {
      "name": "Renhai Peng"
    },
    {
      "name": "Liyan Wang"
    },
    {
      "name": "Ali Raza",
      "orcid": "0000-0002-5120-2791"
    },
    {
      "name": "Bin Jia",
      "orcid": "0000-0002-8358-3613"
    },
    {
      "name": "Zhangli Hu",
      "orcid": "0000-0002-2596-8515"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/asrshz29ab4s8.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-76625-x",
  "fulltext_url": "https://europepmc.org/articles/PMC13687077",
  "id": "asrshz29ab4s8",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://www.nature.com/articles/s41467-026-76625-x",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant biochemistry and biosynthesis"
    ]
  ],
  "openalex": "W7223095394",
  "pdf_url": "https://www.nature.com/articles/s41467-026-76625-x.pdf",
  "pmcid": "PMC13687077",
  "pmid": "42907448",
  "published": "2026-10-21",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/asrshz29ab4s8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:39Z",
      "native_id": "10.1038/s41467-026-76625-x",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41467-026-76625-x"
    },
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42907448",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42907448"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant biochemistry and biosynthesis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Engineering the paclitaxel biosynthetic pathway in Physcomitrium patens for de novo and stable biosynthesis",
  "title_text": "Engineering the paclitaxel biosynthetic pathway in Physcomitrium patens for de novo and stable biosynthesis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/asrshz29ab4s8",
  "venue": "Nature Communications",
  "versions": []
}