{
  "abstract": "Shikimic acid (SA) is a valuable pharmaceutical precursor used in drug synthesis. Here, we present a protocol for isolating and purifying SA from Escherichia coli cultures. We describe steps for combining cell disruption, centrifugation, filtration, preparative anion-exchange chromatography, elution, and lyophilization. We detail procedures for identifying SA-containing fractions by Fourier transform infrared (FTIR) spectroscopy, followed by ethanol-based recrystallization and FTIR characterization of the purified product. This protocol provides an integrated downstream processing strategy for microbial SA production.",
  "abstract_shown": true,
  "affiliations": [
    "LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Pedro Silva"
    },
    {
      "affs": [
        0
      ],
      "name": "Ana C. Afonso"
    },
    {
      "affs": [
        0
      ],
      "name": "Manuel Simões"
    },
    {
      "affs": [
        0
      ],
      "name": "Lúcia Santos"
    },
    {
      "affs": [
        0
      ],
      "name": "Margarida M.S.M. Bastos"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/av2shh55xq05w.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104919",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13734860/",
  "id": "av2shh55xq05w",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104919",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7224012062",
  "pdf_url": "https://www.cell.com/star-protocols/pdf/S2666-1667(26)00572-1.pdf",
  "pmcid": "PMC13734860",
  "pmid": "42945208",
  "published": "2026-10-27",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/av2shh55xq05w.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:20:40Z",
      "native_id": "PMC13734860",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104919"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media",
  "title_text": "Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:20:40Z",
  "url": "https://research.draconitic.com/paper/av2shh55xq05w",
  "venue": "STAR Protocols",
  "versions": []
}