{
  "abstract": "Objective\n\nPancreatic cancer (PC) is highly lethal and resistant to current therapies, underscoring the need for novel strategies that target regulated cell death. This study aimed to evaluate the anti-PC efficacy of total secondary saponins (TSS) from Anemone raddeana and elucidate the mechanism by which TSS induces pyroptosis.Methods\n\nTSS components were characterized by mass spectrometry. Anti-tumor effects were assessed in vitro using human pancreatic cancer cell lines (PANC-1 and AsPC-1 cells) and in vivo using xenograft mouse model. Integrated multi-omics analyses (network pharmacology, proteomics, metabolomics, bioinformatics) identified core components, targets, and pathways. Key findings were validated by molecular docking, immunohistochemistry, and Western blotting.Results\n\nMass spectrometry identified 17 C-3 monosaccharide triterpenoid saponins in TSS. Both in vitro and in vivo models confirmed that TSS can significantly inhibit PC cell proliferation by inducing pyroptosis through the Caspase-3/gasdermin E (GSDME) pathway. Multi-omics highlighted hederacolchiside A1 as a core compound and revealed its regulation of inflammation, proliferation, and oxidative stress through the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) and nuclear factor-κB (NF-κB) signaling pathways. Molecular docking and Western blotting analysis confirmed activation of downstream pyroptosis mechanisms.Conclusion\n\nThis study demonstrates that TSS suppresses PC progression by regulating Caspase-3/GSDME-mediated pyroptosis through modulation of PI3K/Akt and NF-κB signaling pathways, supporting its potential as a novel therapeutic candidate for PC.",
  "abstract_shown": true,
  "affiliations": [
    "School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.",
    "Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China.",
    "Liaoning Provincial Key Laboratory of Traditional Chinnese Medicine Resources Conservation and Development, Shenyang 110016, China.",
    "Liaoning Province Genuine Medicinal Materials Processing Engineering Research Center, Shenyang 110016, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Luanwei Han"
    },
    {
      "affs": [
        0
      ],
      "name": "Mingshu Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Qiao Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Anqi Lu"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Chongning Lv"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Yang Yu"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Jincai Lu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/avf80xy3aepfd.bib",
  "cited_by": 0,
  "doi": "10.1016/j.chmed.2026.06.010",
  "fulltext_url": "https://europepmc.org/articles/PMC13712338",
  "id": "avf80xy3aepfd",
  "issns": [
    "1674-6384",
    "2589-3610"
  ],
  "landing_url": "https://europepmc.org/article/MED/42948729",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1312|Natural product bioactivities and synthesis"
    ]
  ],
  "openalex": "W7166028715",
  "pdf_url": "https://europepmc.org/articles/PMC13712338?pdf=render",
  "pmcid": "PMC13712338",
  "pmid": "42948729",
  "published": "2026-06-26",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/avf80xy3aepfd.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:36Z",
      "native_id": "MED:42948729",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42948729"
    },
    {
      "first_seen": "2026-10-31T12:22:56Z",
      "native_id": "PMC13712338",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.chmed.2026.06.010"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Natural product bioactivities and synthesis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Total secondary saponins from Anemone raddeana suppress pancreatic cancer via Caspase-3/GSDME-mediated pyroptosis.",
  "title_text": "Total secondary saponins from Anemone raddeana suppress pancreatic cancer via Caspase-3/GSDME-mediated pyroptosis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:22:56Z",
  "url": "https://research.draconitic.com/paper/avf80xy3aepfd",
  "venue": "Chinese herbal medicines",
  "versions": []
}